We narrowed to 18,577 results for: MUT

1. pBABEpuro-ARAF

   Plasmid

   #51076

   Purpose

   mammalian expression of wt ARAF

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXL001-TQM IL13 CAR

   Plasmid

   #154054

   Purpose

   Lentiviral expression of TQM IL13 CAR for CAR-T therapy

   Depositor

   Xiaojun Lian

   Article

   Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

   Insert

   TQM IL13 CAR
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG397

   Plasmid

   #226733

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   OMP25 (SYNJ2BP Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-Neo-PTPRT (WT)

   Plasmid

   #16630

   Depositor

   Bert Vogelstein

   Article

   Wang et al Science. 2004 May 21. 304(5674):1164-6.

   Insert

   PTPRT (PTPRT Human)
   

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pRetroX GFP T2A Cre

   Plasmid

   #63704

   Purpose

   Inducible expression of GFP T2A Cre fusion in mammalian cells

   Depositor

   Floris Foijer

   Article

   Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   T2A Cre
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-Rab21-Q76L (CA)

   Plasmid

   #83422

   Purpose

   Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

   Insert

   Rab21 (Rab21 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residue 76 from Q to L (Q76L: CAG -> CTG)

   Promoter

   CMV

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPEY-GAL-Kan-ADE2

   Plasmid

   #232105

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICSL11059

   Plasmid

   #68263

   Purpose

   Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgTrack-BFP

   Plasmid

   #164273

   Purpose

   Empty sgRNA expression vector with co-expression of BFP reporter

   Depositor

   Charles Rudin

   Article

   Wohlhieter et al Cell Rep. 2020 Dec 1;33(9):108444. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EFS

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xMx PylT_GFP 150TAG

    Plasmid

    #140016

    Purpose

    Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG, K46E (Please see depositor comments below)

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-Rab21-T31N (DN)

    Plasmid

    #83423

    Purpose

    Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

    Insert

    Rab21 (Rab21 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Residue 31 from T to N (T31N: ACG -> AAC)

    Promoter

    CMV

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA_355

    Plasmid

    #187159

    Purpose

    Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

    Depositor

    John Doench, David Root

    Article

    Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. VCP(A232E)-EGFP

    Plasmid

    #23973

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    A232E

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTSlb-wt

    Plasmid

    #60728

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZS*12-MS2-GFP

    Plasmid

    #26245

    Depositor

    Philippe Cluzel

    Article

    Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

    Insert

    MS2-GFP
    

    Expression

    Bacterial

    Mutation

    five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

    Available Since

    Nov. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Target-AIDmax

    Plasmid

    #139107

    Purpose

    Mammalian Target-AIDmax expression vector (pSI-626)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-AID-BLM-UGI

    Plasmid

    #221218

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-BLM-UGI
    

    Expression

    Mammalian

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPOP-V5 (BTB)

    Plasmid

    #169721

    Purpose

    Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L186D, L190D, L193D, I217K

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAV1-K*R-mEGFP

    Plasmid

    #27766

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1, lysine null mutant (CAV1 Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    All lysines mutated to arginines (K*R)

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR_L1-YFP-miniTurbo-L2

    Plasmid

    #127352

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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