We narrowed to 8,708 results for: sgRNA

1. pAN-PA1-RFP

   Plasmid

   #62247

   Purpose

   reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pA1

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YN2_1_LT84_RPL13A

   Plasmid

   #177712

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAN-PTet-YFP

   Plasmid

   #62245

   Purpose

   used to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAN-PA3-RFP

   Plasmid

   #62249

   Purpose

   reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pA3

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBC2101-reci

   Plasmid

   #117170

   Purpose

   U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   reci_wtCas9 with SV40 NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAN-PBAD-YFP

   Plasmid

   #62246

   Purpose

   used to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TLCV2 - sgAP2S1 #2

   Plasmid

   #202757

   Purpose

   Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

   Depositor

   Christopher Counter

   Article

   Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

   Insert

   gRNA targeting AP2S1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TLCV2 - sgAP2M1

   Plasmid

   #202758

   Purpose

   Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2M1

   Depositor

   Christopher Counter

   Article

   Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

   Insert

   gRNA targeting AP2M1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TLCV2 - sgAP2S1 #1

   Plasmid

   #202756

   Purpose

   Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

   Depositor

   Christopher Counter

   Article

   Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

   Insert

   gRNA targeting AP2S1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psBIG1a ActBT2ATetP2AP - mAGH

    Plasmid

    #229973

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - mAGH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psBIG1a ActBT2ATetP2AP - H

    Plasmid

    #229972

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - H
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psBIG1a ActBT2AmcherryP2AP -TagBFPH

    Plasmid

    #229971

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2AmcherryP2AP -TagBFPH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEVA47_dCas9-GFP_NOT_i1

    Plasmid

    #231416

    Purpose

    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    dCas9
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHPHS309

    Plasmid

    #228230

    Purpose

    GFP dropout backbone for sgRNA library cloning

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CMV-dSa VP64 Afp

    Plasmid

    #99697

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpy

    Plasmid

    #220184

    Purpose

    sgRNA expression vector - promoterless backbone

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    promoterless backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    promoterless

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpyA

    Plasmid

    #220185

    Purpose

    sgRNA expression vector - pEM7 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pEM7 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pEM7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpyC

    Plasmid

    #220187

    Purpose

    sgRNA expression vector - pBG42 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBG42

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpyD

    Plasmid

    #220188

    Purpose

    sgRNA expression vector - pBG28 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG28 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBG28

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBH504

    Plasmid

    #215782

    Purpose

    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene

    Depositor

    Corey Wilson

    Article

    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

    Inserts

    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bt1311, P(O1), and P1

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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