We narrowed to 561 results for: BIG;

1. pBIG1a-PAF1CdeltaCDC73

   Plasmid

   #231746

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CTR9 (CTR9 Human)
   PAF1 (PAF1 Human)
   LEO1 (LEO1 Human)
   WDR61 (SKIC8 Human)
   

   Tags

   3C-Strep(II)

   Expression

   Insect

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG1a-PAF1CdeltaLEO1

   Plasmid

   #231747

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CTR9 (CTR9 Human)
   CDC73 (CDC73 Human)
   PAF1 (PAF1 Human)
   WDR61 (SKIC8 Human)
   

   Tags

   3C-Strep(II) and Flag-3C

   Expression

   Insect

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. psBIG1a ActBT2ATetP2AP - H

   Plasmid

   #229972

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2ATetP2AP - H
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR/hBIG1

   Plasmid

   #226251

   Purpose

   Entry plasmid of hBIG1 for Gateway system

   Depositor

   Hye-Won Shin

   Insert

   BIG1 (ARFGEF1 Human)
   

   Use

   Entry vector

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR/HA-hBIG2

   Plasmid

   #226252

   Purpose

   Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system

   Depositor

   Hye-Won Shin

   Insert

   BIG2 (ARFGEF2 Human)
   

   Use

   Entry vector

   Tags

   HA

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIG1a_StrepII-Hook3_FTS_FHIP1B

   Plasmid

   #222299

   Purpose

   Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3 (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Tags

   StrepII-Psc

   Expression

   Bacterial

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBIG1a_StrepII-Hook2_FTS_FHIP2A

   Plasmid

   #222302

   Purpose

   Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK2 (HOOK2 Human)
   FTS (AKTIP Human)
   FHIP2A (FHIP2A Human)
   

   Tags

   StrepII-Psc

   Expression

   Insect

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-Embigin

   Plasmid

   #221417

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   Embigin (EMB Human)
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT22gCfpUbiG4FER4P2A(#249)

   Plasmid

   #184070

   Purpose

   cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

   Insert

   Gal4bdFF-ERT2-P2A
   

   Use

   Zebrafish transgenesis (blue eyes)

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-Bighead S-FC

    Plasmid

    #180147

    Purpose

    mRNA synthesis for injection into embryos

    Depositor

    Edward De Robertis

    Article

    Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

    Insert

    Bighead.S
    

    Tags

    FC

    Expression

    Mammalian

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2 Bighead S-HA

    Plasmid

    #172485

    Purpose

    for synthesis of mRNA for injection into xenopus laevis embryos

    Depositor

    Edward De Robertis

    Article

    Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

    Insert

    Bighead.S
    

    Use

    In vitro mrna synthesis

    Tags

    HA and S-HA

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG1e-CDK9/Cyclin T1

    Plasmid

    #231732

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK9 (CDK9 Human)
    CCNT1 (CCNT1 Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRubiG-T2A-Cas9

    Plasmid

    #75348

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBIG1e-CDK12deltaRS/Cyclin K

    Plasmid

    #231744

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(delta104-380)

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBIG1e-CDK12/Cyclin K

    Plasmid

    #231730

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBIG1e-CDK13/Cyclin K

    Plasmid

    #231731

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK13 (CDK13 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. CBIG-Sox5 variant 3

    Plasmid

    #48707

    Purpose

    bicistronic expression of Sox5 and EGFP

    Depositor

    Jeffrey Macklis

    Insert

    Sox5 (Sox5 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    variant 3

    Promoter

    CMV, beta-actin

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRubiG-T2A-cre

    Plasmid

    #66693

    Purpose

    Recombine floxed alleles in newborn neurons

    Depositor

    Bryan Luikart

    Article

    Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

    Insert

    GFP-T2A-cre
    

    Use

    Retroviral

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pbIG-Xph20-ETWV

    Plasmid

    #133034

    Purpose

    Expresses Xph20-ETWV in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-ETWV
    

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBIG1e-CDK12deltaNTD /Cyclin K

    Plasmid

    #231741

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(700-1490)

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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