We narrowed to 116 results for: Toxoplasma gondii

1. pLinker-AID-3xHA-DHFR-LoxP

   Plasmid

   #86669

   Purpose

   for generation of AID strains using CRISPR tagging technology

   Depositor

   David Sibley

   Article

   Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

   Insert

   Linker-AID-3xHA-HXGPRT 3'UTR
   

   Use

   Expression in toxoplasma gondii

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLinker-AID-Ty-HXGPRT-LoxP

   Plasmid

   #86667

   Purpose

   for generation of AID strains using CRISPR tagging technology

   Depositor

   David Sibley

   Article

   Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

   Insert

   Linker-AID-Ty-HGPRT 3'UTR
   

   Use

   Expression in toxoplasma gondii

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ATSR

   Plasmid

   #50052

   Purpose

   expresses t.gondii VWA+TSR1 domains in mammalian cells

   Depositor

   Timothy Springer

   Insert

   MIC2-ATSR
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   S158A

   Promoter

   Chick beta-actin promoter

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. proATSR

   Plasmid

   #50053

   Purpose

   Expresses T.gondii prodomain+VWA+TSR1 domains in mammalian cells

   Depositor

   Timothy Springer

   Insert

   MIC2-proATSR
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   S158A

   Promoter

   chick beta-actin promoter

   Available since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

5. PP-ROP2A

   Plasmid

   #25130

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   33.m01398:C43601
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. A5TSR

   Plasmid

   #50358

   Purpose

   expresses T.gondii MIC2(67-593) domains in mammalian cells

   Depositor

   Timothy Springer

   Insert

   MIC2-A5TSR
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   S158A, N357S, N463S, N470D

   Promoter

   CHICK beta-actin promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

7. A6TSR

   Plasmid

   #50359

   Purpose

   expresses T.gondii MIC2(67-660) domains in mammalian cells

   Depositor

   Timothy Springer

   Insert

   MIC2-A6TSR
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   S158A, N357S, N463S, N470D

   Promoter

   CHICK beta-actin promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

8. PP-mACP

   Plasmid

   #25124

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   59.m03510:C40207
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pLinker-BirA-3xHA-HXGPRT-loxP

   Plasmid

   #86668

   Purpose

   for generation of BirA tagged strains using CRISPR tagging technology

   Depositor

   David Sibley

   Insert

   Linker-BirA-3xHA-HXGPRT 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PP-ROP8

    Plasmid

    #25123

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    33.m00005:C33888 (TGME49_039600 Toxoplasma gondii)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. PP-CyP8

    Plasmid

    #25122

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    541.m00136:C37419
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. Tg-UBC (TgTwinScan_2218)

    Plasmid

    #25565

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Tg-UBC (TgTwinScan_2218)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. PP-CyP5

    Plasmid

    #25121

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    49.m03261:C37453
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pUPRT::DHFR-D

    Plasmid

    #58528

    Purpose

    replace TgUPRT with DHFR by homologous recombination

    Depositor

    David Sibley

    Article

    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

    Insert

    DHFR cassette
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    pyrimethamine resistant

    Promoter

    DHFR

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MIC2

    Plasmid

    #50050

    Purpose

    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells

    Depositor

    Timothy Springer

    Insert

    MIC2
    

    Use

    Tags

    HIS, SBP, and TEV

    Expression

    Mammalian

    Mutation

    S158A, N357S, N463S, N470D

    Promoter

    CMV

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. m2ap

    Plasmid

    #50051

    Purpose

    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells

    Depositor

    Timothy Springer

    Insert

    M2AP
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    starts at aa47; N127R, S158A, S177N, N357S, N463S…

    Promoter

    CMV

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. PP-CDPK4 (3MA6)

    Plasmid

    #25275

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TgCDPK4:P36-Q315
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids P36-Q315

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. PP-PYRK1 (3GG8)

    Plasmid

    #25288

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    55.m00007:I39-E531
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids I39-E531

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. PP-ACP

    Plasmid

    #25188

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    55.m00019:s:S100-S180
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 9, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. PP-CDPK1 (3HZT)

    Plasmid

    #25189

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    541.m00134:L72-H537
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids L72-H537

    Promoter

    Available since

    Aug. 9, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits