We narrowed to 100 results for: Toxoplasma gondii
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Plasmid#78705PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable markerDepositorInsertGCaMP5
PromoterSAG1 5' UTRAvailable sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRPromoterDHFR-TS 5' UTRAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…PromoterRPS13 with Tet Operators InsertedAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
m2ap
Plasmid#50051Purposeexpresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cellsDepositorInsertM2AP
TagsHISExpressionMammalianMutationstarts at aa47; N127R, S158A, S177N, N357S, N463S…PromoterCMVAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGCaMP5-DHFR
Plasmid#78706PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable marker.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsGCaMP5
DHFR
PromoterToxoplasma SAG1 5' UTRAvailable sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
m2ap
Plasmid#50051Purposeexpresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cellsDepositorInsertM2AP
TagsHISExpressionMammalianMutationstarts at aa47; N127R, S158A, S177N, N357S, N463S…PromoterCMVAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCvL31-NanoLuc^opt
Plasmid#255782PurposeExpresses NanoLuc luciferase (codon optimized for Chromera) in Chromera veliaDepositorInsertCv β-tubulin intron 1 + GAPDH Kozak + NanoLuc
UseChromera velia, toxoplasma gondiiExpressionBacterialMutationcodon optimized for Chromera veliaPromoterputative C. velia L31 promoter (1103 bp)Available sinceJune 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBS-HA::UPRT
Plasmid#47110Purposefor subcloning UPRT into expression vectorsDepositorInserturacil phosphoribosyltransferase
TagsHAAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS-HA::UPRT
Plasmid#47110Purposefor subcloning UPRT into expression vectorsDepositorInserturacil phosphoribosyltransferase
TagsHAAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-Decoy
Plasmid#80324PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondiiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDecoy sgRNA
UseCRISPRPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lourido Toxoplasma CRISPR Library V1
Pooled library#80636PurposeKnockout library targeting 8,158 predicted protein-coding gene in Toxoplasma gondii.DepositorAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSAG1 sgRNA
UseCRISPRPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYFP-TetR
UseToxoplasma expressionPromoteralpha-tubulinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYFP-TetR
UseToxoplasma expressionPromoteralpha-tubulinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMVtight-UPRT-T2A-RFP-IRES-CD
Plasmid#126677PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
Promotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLinker-6xHA-HXGPRT-LoxP
Plasmid#86552PurposeThe plasmid is used for endogenous tagging using CRISPR technology in ToxoplasmaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLinker-6xHA-HGPRT-LoxP
UseExpression in toxoplasma gondiiAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLinker-2xTy-HXGPRT-LoxP
Plasmid#86664PurposeCRISPR tagging with 2xTy in Toxoplamsa gondiiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLinker-2xTy-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLinker-BirA-3xHA-HXGPRT-loxP
Plasmid#86668Purposefor generation of BirA tagged strains using CRISPR tagging technologyDepositorInsertLinker-BirA-3xHA-HXGPRT 3'UTR
ExpressionBacterialAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFloxed DHFR-TS*
Plasmid#100605PurposepUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTRDepositorInsertFloxed TgDHFR-TS*
UseToxoplasma expressionMutationPyrimethamine resistanceAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tagger
Plasmid#129396PurposeExpresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoterDepositorInsertrpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
TagsFLAG, HA, and V5ExpressionMammalianPromoterCMVAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only