We narrowed to 1,029 results for: gibson assembly

1. TOPO CMV

   Plasmid

   #68454

   Purpose

   TOPO construct expressing CMV for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Cytomegalovirus Immediate-Early Promoter
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPWCh

   Plasmid

   #124238

   Purpose

   Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.

   Depositor

   Peter Vilmos

   Article

   Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UASp

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET26b(modified)-Lg-B

   Plasmid

   #216960

   Purpose

   Expression of Large BiT-Linker-GvpB

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Large BiT-Linker-GvpB
   

   Use

   Tags

   FLAG-Split Nanoluciferase LargeBiT

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET26b(modified)-B-Lg

   Plasmid

   #216959

   Purpose

   Expression of GvpB-Linker-Large BiT

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   GvpB-Linker-Large BiT
   

   Use

   Tags

   Split Nanoluciferase LargeBiT-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST39-Mega'

   Plasmid

   #216877

   Purpose

   Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Derived from Mega, and GvpS and GvpK overlap has been resolved
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-H2-18 Heavy chain-SpyTag003

   Plasmid

   #216307

   Purpose

   Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Tras heavy chain-SpyTag003

   Plasmid

   #216293

   Purpose

   Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TR knockout HRD

   Plasmid

   #207579

   Purpose

   Homologous recombination donor replacing TR with a puro cassette.

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   puro resistance cassette flanked by TR locus sequences excluding TR
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. nUnaG-ST

   Plasmid

   #207630

   Purpose

   A plasmid encoding the N-terminal UnaG fragment fused to SpyTag.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   nUnaG-SpyTag-MBP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 1-84

   Promoter

   T7

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL0_DE_mTurquoise2

    Plasmid

    #202155

    Purpose

    Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    mTurquoise2 gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCA_e-4

    Plasmid

    #202099

    Purpose

    Receiver cloning vector for even-4 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCA_e-3

    Plasmid

    #202098

    Purpose

    Receiver cloning vector for even-3 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCA_e-1

    Plasmid

    #202096

    Purpose

    Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCA_o-4

    Plasmid

    #202094

    Purpose

    Receiver cloning vector for odd-4 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCA_o-3

    Plasmid

    #202093

    Purpose

    Receiver cloning vector for odd-3 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCA_o-2

    Plasmid

    #202092

    Purpose

    Receiver cloning vector for odd-2 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pCA_o-1

    Plasmid

    #202091

    Purpose

    Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pBN086

    Plasmid

    #173448

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y715E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y715E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y715E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBN087

    Plasmid

    #173449

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y717E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y717E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y717E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBN088

    Plasmid

    #173450

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y718E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y718E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge