We narrowed to 1,062 results for: gibson assembly

1. TOPO SV40

   Plasmid

   #68455

   Purpose

   TOPO construct expressing SV40 for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Simian Virus Early 40 Promoter
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNS3

   Plasmid

   #131770

   Purpose

   PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
   

   Use

   Integration into δ-sites

   Tags

   Expression

   Yeast

   Mutation

   E490G to make Cry2 into Cry2Olig

   Promoter

   PADH1

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNS1

   Plasmid

   #131769

   Purpose

   PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   FUSN_Fusion Red_Cry2WT
   

   Use

   Integration into δ-sites

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PADH1

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FFiBlast MSCV Puro

   Plasmid

   #68464

   Purpose

   Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Blasticidin Resistance
   

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Inverted

   Promoter

   LTR

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. STAGR_SAMScaffold_hH1

   Plasmid

   #102847

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_hH1
   

   Use

   Pcr template for stagr inserts

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. STAGR_SAMScaffold_h7SK

   Plasmid

   #102846

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_h7SK
   

   Use

   Pcr template for stagr inserts

   Tags

   Expression

   Mutation

   Promoter

   H7SK

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOPO CMV

   Plasmid

   #68454

   Purpose

   TOPO construct expressing CMV for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Cytomegalovirus Immediate-Early Promoter
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPWCh

   Plasmid

   #124238

   Purpose

   Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.

   Depositor

   Peter Vilmos

   Article

   Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UASp

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET26b(modified)-Lg-B

   Plasmid

   #216960

   Purpose

   Expression of Large BiT-Linker-GvpB

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Large BiT-Linker-GvpB
   

   Use

   Tags

   FLAG-Split Nanoluciferase LargeBiT

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET26b(modified)-B-Lg

    Plasmid

    #216959

    Purpose

    Expression of GvpB-Linker-Large BiT

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    GvpB-Linker-Large BiT
    

    Use

    Tags

    Split Nanoluciferase LargeBiT-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST39-Mega'

    Plasmid

    #216877

    Purpose

    Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Derived from Mega, and GvpS and GvpK overlap has been resolved
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-H2-18 Heavy chain-SpyTag003

    Plasmid

    #216307

    Purpose

    Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Tras heavy chain-SpyTag003

    Plasmid

    #216293

    Purpose

    Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TR knockout HRD

    Plasmid

    #207579

    Purpose

    Homologous recombination donor replacing TR with a puro cassette.

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    puro resistance cassette flanked by TR locus sequences excluding TR
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. nUnaG-ST

    Plasmid

    #207630

    Purpose

    A plasmid encoding the N-terminal UnaG fragment fused to SpyTag.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    nUnaG-SpyTag-MBP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 1-84

    Promoter

    T7

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL0_DE_mTurquoise2

    Plasmid

    #202155

    Purpose

    Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    mTurquoise2 gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCA_e-4

    Plasmid

    #202099

    Purpose

    Receiver cloning vector for even-4 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCA_e-3

    Plasmid

    #202098

    Purpose

    Receiver cloning vector for even-3 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pBN086

    Plasmid

    #173448

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y715E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y715E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y715E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBN087

    Plasmid

    #173449

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y717E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y717E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y717E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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