We narrowed to 1,086 results for: gibson assembly

1. MSCV Puro-SV40:GFP

   Plasmid

   #68483

   Purpose

   Retrovirus expressing GFP driven by SV40 promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR and SV40

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TOPO EFS

   Plasmid

   #68451

   Purpose

   TOPO construct expressing EFS for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Elongation Factor 1α Promoter
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV Puro-EFS:GFP

   Plasmid

   #68484

   Purpose

   Retrovirus expressing GFP driven by EFS promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   EFS and LTR

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. TOPO CCSP

   Plasmid

   #68453

   Purpose

   TOPO construct expressing CCSP for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Clara Cell Secretory Protein Promoter
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNS3

   Plasmid

   #131770

   Purpose

   PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
   

   Use

   Integration into δ-sites

   Expression

   Yeast

   Mutation

   E490G to make Cry2 into Cry2Olig

   Promoter

   PADH1

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNS1

   Plasmid

   #131769

   Purpose

   PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   FUSN_Fusion Red_Cry2WT
   

   Use

   Integration into δ-sites

   Expression

   Yeast

   Promoter

   PADH1

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOPO SV40

   Plasmid

   #68455

   Purpose

   TOPO construct expressing SV40 for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Simian Virus Early 40 Promoter
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FFiBlast MSCV Puro

   Plasmid

   #68464

   Purpose

   Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Blasticidin Resistance
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Mutation

   Inverted

   Promoter

   LTR

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. STAGR_SAMScaffold_hH1

   Plasmid

   #102847

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_hH1
   

   Use

   Pcr template for stagr inserts

   Promoter

   U6

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. STAGR_SAMScaffold_h7SK

    Plasmid

    #102846

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_h7SK
    

    Use

    Pcr template for stagr inserts

    Promoter

    H7SK

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TOPO CMV

    Plasmid

    #68454

    Purpose

    TOPO construct expressing CMV for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Cytomegalovirus Immediate-Early Promoter
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPWCh

    Plasmid

    #124238

    Purpose

    Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.

    Depositor

    Peter Vilmos

    Article

    Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    UASp

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-Puro-EGFP

    Plasmid

    #246560

    Purpose

    Retroviral expression vector for EGFP

    Depositor

    Mario Vallon

    Article

    Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

    Insert

    GFP
    

    Use

    Retroviral

    Mutation

    F64L, S65T (EGFP)

    Promoter

    MMLV LTR

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. EBFP2-Kindlin1

    Plasmid

    #242821

    Purpose

    Encodes wildtype human kindlin1 and N-terminal EBFP2 tag

    Depositor

    Cheng-han Yu

    Article

    Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

    Insert

    Kindlin1 (Fermt1 Human)
    

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-ARHGEF6

    Plasmid

    #242830

    Purpose

    Encodes human ARHGEF6 and N-terminal EGFP tag

    Depositor

    Cheng-han Yu

    Article

    Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

    Insert

    ARHGEF6 (ARHGEF6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET26b(modified)-Lg-B

    Plasmid

    #216960

    Purpose

    Expression of Large BiT-Linker-GvpB

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Large BiT-Linker-GvpB
    

    Tags

    FLAG-Split Nanoluciferase LargeBiT

    Expression

    Bacterial

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET26b(modified)-B-Lg

    Plasmid

    #216959

    Purpose

    Expression of GvpB-Linker-Large BiT

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    GvpB-Linker-Large BiT
    

    Tags

    Split Nanoluciferase LargeBiT-FLAG

    Expression

    Bacterial

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST39-Mega'

    Plasmid

    #216877

    Purpose

    Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Derived from Mega, and GvpS and GvpK overlap has been resolved
    

    Expression

    Bacterial

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Tras heavy chain-SpyTag003

    Plasmid

    #216293

    Purpose

    Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TR knockout HRD

    Plasmid

    #207579

    Purpose

    Homologous recombination donor replacing TR with a puro cassette.

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    puro resistance cassette flanked by TR locus sequences excluding TR
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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