We narrowed to 1,107 results for: gibson assembly
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Plasmid#216304PurposeExpresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras heavy chain-SpyTag003
Plasmid#216293PurposeExpresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink FabDepositorInsertTras heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Heavy chain-SpyTag003
Plasmid#216296PurposeExpresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S FabDepositorInsert39S Heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFP.R274
Plasmid#138223PurposeExpresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag DK
Plasmid#112635PurposeSpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DKDepositorInsertSpyTag-ELP-SpyTag DK
TagsHis6ExpressionBacterialMutationContains central cysteine. Reactive aspartic acid…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-yomRuby3-SpHIS5
Plasmid#204287PurposeFor C-terminal tagging using yeast optimized mRuby3DepositorInsertpFA6-yomRuby3-SpHIS5
TagsyomRuby3ExpressionYeastPromoternoneAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His-ShadowG-iLID
Plasmid#246942PurposeExpresses His-ShadowG-iLID in E.coliDepositorInsertiLiD
TagsHis-tag and ShadowGExpressionBacterialAvailable SinceApril 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSuper TR G414C
Plasmid#207612PurposeU3 promoter driven expression of the telomerase RNA with a G414C CAB box mutationDepositorInsertTelomerase RNA G414C
ExpressionMammalianPromoterU1Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
Tags3xMS2ExpressionMammalianPromoterSV40Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-T-Lg
Plasmid#216977PurposeExpression of GvpT-Linker-Large BiTDepositorInsertGvpT-Linker-Large BiT
TagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaRLS
Plasmid#216955PurposeMega' with knockout of GvpR, GvpL and GvpSDepositorInsertMega' with knockout of GvpR, GvpL and GvpS
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBSU
Plasmid#216953PurposeMega' with knockout of GvpB, GvpS and GvpUDepositorInsertMega' with knockout of GvpB, GvpS and GvpU
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaLSK
Plasmid#216957PurposeMega' with knockout of GvpL, GvpS and GvpKDepositorInsertMega' with knockout of GvpL, GvpS and GvpK
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBRG
Plasmid#216945PurposeMega' with knockout of GvpB, GvpR and GvpGDepositorInsertMega' with knockout of GvpB, GvpR and GvpG
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMulti_K_ccdB
Plasmid#223852PurposeGEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)DepositorTypeEmpty backboneUseGolden-gate acceptorExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMulti_A_ccdB
Plasmid#223851PurposeGEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)DepositorTypeEmpty backboneUseGolden-gate acceptorExpressionBacterialAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-Sm-U
Plasmid#217003PurposeExpression of Small BiT-Linker-GvpUDepositorInsertSmall BiT-Linker-GvpU
TagsSplit Nanoluciferase SmallBiTExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBRS
Plasmid#216947PurposeMega' with knockout of GvpB, GvpR and GvpSDepositorInsertMega' with knockout of GvpB, GvpR and GvpS
ExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBLJ
Plasmid#216951PurposeMega' with knockout of GvpB, GvpL and GvpJDepositorInsertMega' with knockout of GvpB, GvpL and GvpJ
ExpressionBacterialAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-G-Sm
Plasmid#216990PurposeExpression of GvpG-Linker-Small BiTDepositorInsertGvpG-Linker-Small BiT
TagsSplit Nanoluciferase SmallBiTExpressionBacterialAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only