pQE80L SpyTag-ELP-SpyTag DK
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||112635||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerQiagen Inc.
- Backbone size w/o insert (bp) 4714
- Total vector size (bp) 5393
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSpyTag-ELP-SpyTag DK
Insert Size (bp)690
MutationContains central cysteine. Reactive aspartic acid in C-terminal SpyTag mutated to lysine (DK).
- Promoter T5
/ Fusion Protein
- His6 (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer CATAAAAAATTTATTTGCTTTGTGAGCGG
- 3′ sequencing primer CGAGCGTTCTGAACAAATCC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQE80L SpyTag-ELP-SpyTag DK was a gift from Mark Howarth (Addgene plasmid # 112635 ; http://n2t.net/addgene:112635 ; RRID:Addgene_112635)
For your References section:Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions. Wieduwild R, Howarth M. Biomaterials /10.1016/j.biomaterials.2018.07.020