pQE80L SpyTag-ELP-SpyTag DK
(Plasmid #112635)

• Purpose: SpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DK
• Depositing Lab: Mark Howarth
• Publication: Wieduwild et al Biomaterials

Backbone

• Vector backbone: pQE80L
  (Search Vector Database)
• Backbone manufacturer: Qiagen Inc.
• Backbone size w/o insert (bp): 4714
• Total vector size (bp): 5393
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyTag-ELP-SpyTag DK
• Species: Synthetic
• Insert Size (bp): 690
• Mutation: Contains central cysteine. Reactive aspartic acid in C-terminal SpyTag mutated to lysine (DK).
• Promoter: T5
• Tag / Fusion Protein:
  • His6 (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CATAAAAAATTTATTTGCTTTGTGAGCGG
• 3′ sequencing primer: CGAGCGTTCTGAACAAATCC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry