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pQE80L SpyTag-ELP-SpyTag DK
(Plasmid #112635)


Item Catalog # Description Quantity Price (USD)
Plasmid 112635 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Qiagen Inc.
  • Backbone size w/o insert (bp) 4714
  • Total vector size (bp) 5393
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    SpyTag-ELP-SpyTag DK
  • Species
  • Insert Size (bp)
  • Mutation
    Contains central cysteine. Reactive aspartic acid in C-terminal SpyTag mutated to lysine (DK).
  • Promoter T5
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 3′ sequencing primer CGAGCGTTCTGAACAAATCC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE80L SpyTag-ELP-SpyTag DK was a gift from Mark Howarth (Addgene plasmid # 112635 ; ; RRID:Addgene_112635)
  • For your References section:

    Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions. Wieduwild R, Howarth M. Biomaterials /10.1016/j.biomaterials.2018.07.020