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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,576 results for: Vars;

Showing: 5981 - 6000 of 11576 results

  1. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid
    #170240
    Purpose
    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    S218D, S222D
    Promoter
    EF1a
    Available Since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170240-plasmid-map-sequence-id-334554

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  8. pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

    Plasmid
    #106206
    Purpose
    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: S72A + mRuby3 fusion
    Promoter
    CAG
    Available Since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106206-plasmid-map-sequence-id-201033

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  10. pEMS1424

    Plasmid
    #29220
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available Since
    Nov. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
    29220-plasmid-map-sequence-id-13635

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  13. pAAV.CAG.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106202
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available Since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    106202-plasmid-map-sequence-id-204609

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  20. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139088
    Purpose
    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9 3.7
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    A262T, R324L,S409I, E480K, E543D, M694I, E1219V
    Promoter
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139088-plasmid-map-sequence-id-273884

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Showing: 5981 - 6000 of 11576 results