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We narrowed to 7,548 results for: ski

Showing: 5981 - 6000 of 7548 results

  3. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid
    #204215
    Purpose
    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.
    Depositor
    Guei-Sheung Liu
    Article
    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121
    Insert
    Cas13bt3 and U6.SapI sgRNA cloning site
    Use
    AAV and CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Codon optimisation by GenScript tool
    Promoter
    CMV/U6
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    204215-plasmid-map-sequence-id-455913

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  7. Cherry-HA-KCC2

    Plasmid
    #104077
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Use
    Tags
    mCherry-HA tag
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104077-plasmid-map-sequence-id-199357

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  8. pMXs-E55A-ATPIF1

    Plasmid
    #85404
    Purpose
    Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase
    Depositor
    David Sabatini
    Article
    Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1
    Insert
    ATPIF1 (ATP5IF1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    E55A point mutation abrogating the ability of ATP…
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. B2M Bulldozer (gRNA crB2M_13)

    Plasmid
    #84381
    Purpose
    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells
    Depositor
    Chad Cowan
    Article
    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.
    Insert
    B2M Bulldozer crB2M_13 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84381-plasmid-map-sequence-id-189203

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  18. t1-405

    Plasmid
    #166127
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    Tln1Head (1-405) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405 only
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166127-plasmid-map-sequence-id-330252

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  19. pAAV.hSynap.iAChSnFR-NULL

    Plasmid
    #137951
    Purpose
    Expresses iAChSnFR (non-binding) under hSynapsin promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (non-binding variant)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Y140A
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137951-plasmid-map-sequence-id-271588

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Showing: 5981 - 6000 of 7548 results