We narrowed to 14,835 results for: NOL

1. AA246

   Plasmid

   #215946

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD55_v1 [Sp]; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AA233

   Plasmid

   #215941

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AA244

   Plasmid

   #215945

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AA242

   Plasmid

   #215943

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD47_v1 [Sp]; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AA243

   Plasmid

   #215944

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD47_v5 [Sp]; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AA310

   Plasmid

   #215950

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control lethal

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgPLK1_v2 [Sp]; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AA232

   Plasmid

   #215940

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD47_v4 [Sp]; trRNA_v14 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AA238

   Plasmid

   #215942

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD47_v4 [Sp]; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AA054

   Plasmid

   #215932

   Purpose

   Fragmid fragment: (guide cassette) guide expression; reverse orientation

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3}
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AA183

    Plasmid

    #216025

    Purpose

    Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9 (H840A)_v2.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AA285

    Plasmid

    #215947

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD81_v1; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AA023

    Plasmid

    #215931

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD81_v1 [Sp]; trRNA_v2 [Sp]; CS2
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AA102

    Plasmid

    #215935

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AA107

    Plasmid

    #215936

    Purpose

    Fragmid fragment: (guide cassette) guide expression; enables iBar UMIs

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMV_AA091

    Plasmid

    #216106

    Purpose

    CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gRNA expression with modified trRNA to recruit activators via PP7 tags
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-DONSON-DEWS

    Plasmid

    #208799

    Purpose

    Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    D374A; E377A; W381A; S437A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-DONSON-3A

    Plasmid

    #208800

    Purpose

    Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    V473A; R476A; Y481A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-DONSON-Δ24

    Plasmid

    #208801

    Purpose

    Xenopus laevis His6-Myc-DONSON (1-24aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Δ24, deletion of 1-24aa

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-DONSON-DEW

    Plasmid

    #208802

    Purpose

    Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    D374A; E377A; W381A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Qt-FLAG-TGA-rt9us-antiGFP

    Plasmid

    #206954

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLINE1mORF1-ΔORF2-scFvFc

    Plasmid

    #206023

    Purpose

    The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Qt-FLAG-TAA-rt20s-antiGFP

    Plasmid

    #206955

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 1M-Qt-FLAG-TGA-rt20s-antiGFP

    Plasmid

    #206953

    Purpose

    Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET19-TdTdelta5cys

    Plasmid

    #207465

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTdelta5cys (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-CAG-BR1-P2A-FusionRed

    Plasmid

    #192819

    Purpose

    Expresses BR1 with FusionRed tag in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    BR1 (BRI1 Mustard Weed)
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. B*46:01:01

    Plasmid

    #203250

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*46:01:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. B*53:01:01

    Plasmid

    #203249

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*53:01:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. B*08:01:01

    Plasmid

    #203251

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*08:01:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. B*07:04

    Plasmid

    #203244

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*07:04 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. A*26:01:01

    Plasmid

    #203285

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*26:01:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge