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JC001: pMVP/Ad/RGD-DEST

Plasmid

#121844

Purpose

pMVP destination vector, empty RGD fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12g1-noncoding

Plasmid

#120880

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12g1

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCph8

Plasmid

#22869

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2005 Nov 24. 438(7067):441-2.

Insert

pCph8

Use

Synthetic Biology

Expression

Bacterial

Mutation

First 517 amino acids of Cph1 and the last 229 am…

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNA500

Plasmid

#149583

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRKB134_GI-DUX4-intron2(-)

Plasmid

#83968

Purpose

to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

Depositor

Robert Bradley

Article

Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

Insert

DUX4-3'UTR-intron2(-) (DUX4 Human)

Expression

Mammalian

Promoter

mouseCMV

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pRKB133_GI-DUX4-intron2(+)

Plasmid

#83967

Purpose

to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

Depositor

Robert Bradley

Article

Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

Insert

DUX4-3'UTR-intron2(+) (DUX4 Human)

Expression

Mammalian

Promoter

mouseCMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Plexin D1 in situ probe

Plasmid

#45621

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Plexin D1 in situ probe (Plxnd1 Mouse)

Use

In situ

Mutation

fragment contains bp# 5751-6406 of NM_026376.3

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad2 (100-467)

Plasmid

#14928

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (100-467) (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

amino acids 100-467

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

Plasmid

#62944

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad2 (1-185)

Plasmid

#14929

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (1-185) (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

amino acids 1-185

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

Plasmid

#62946

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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KN206: pMVP/eCFP-DEST

Plasmid

#121854

Purpose

pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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tdEos-VSVG-16

Plasmid

#57693

Purpose

Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VSVG

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pJet-CMV-CD5Flag2-bglpA

Plasmid

#89705

Purpose

Expression plasmid for Flag embedded into human CD52 with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CD5Flag2-bglpA

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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JH401: pMVP/Ad/RGD-PK-DEST

Plasmid

#121846

Purpose

pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad2 (1-456)

Plasmid

#14958

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-456 (tail deletion)

Promoter

CMV

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-SFXN3

Plasmid

#110637

Purpose

Retroviral expression of Flag-SFXN3 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN3 (SFXN3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78306

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-V5-NLGN2

Plasmid

#82538

Purpose

pCAG vector for expressing V5-NLGN2

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

NLGN2 (Nlgn2 Rat)

Tags

V5

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pACYC-Cas12c2-noncoding

Plasmid

#120877

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12c2

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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