We narrowed to 59,538 results for: cloning

1. pICH51288

   Plasmid

   #50269

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. TB:4xHRE_RE:MiniP

   Plasmid

   #178313

   Purpose

   4 copies of the HRE DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   4 copies of the heat shock DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_124

   Plasmid

   #133461

   Purpose

   EF1a promoter expresses SpyoCas9 and P2A provides EGFP

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   SpyoCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYSD099

   Plasmid

   #212923

   Purpose

   A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCCl-MCS_miniCMV_TagBFP

   Plasmid

   #134986

   Purpose

   The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

   Depositor

   Claudio Mussolino

   Article

   Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

   Insert

   TagBFP
   

   Use

   Lentiviral

   Promoter

   minCMV

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRL1383a

   Plasmid

   #70692

   Purpose

   RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

   Depositor

   Peter Wolk

   Article

   Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

   Type

   Empty backbone

   Use

   Bacterial shuttle vector

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_BIRC6

   Plasmid

   #197974

   Purpose

   Entry clone for wt BIRC6

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Use

   Other

   Mutation

   L1332V

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIS3

   Plasmid

   #92173

   Purpose

   Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

   Depositor

   Judith Berman

   Article

   Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

   Insert

   NAT1
   

   Use

   Genbank: u03440.1

   Expression

   Yeast

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWBC

   Plasmid

   #15239

   Depositor

   Ron Vale

   Article

   Niclas et al Neuron. 1994 May . 12(5):1059-72.

   Insert

   Human neuronal kinesin heavy chain (KIF5A Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pLARRYv2-mScarlet

    Plasmid

    #233210

    Purpose

    Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with mScarlet marker

    Depositor

    Alejo Rodriguez-Fraticelli

    Article

    Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLARRYv2-T-Sapphire

    Plasmid

    #233209

    Purpose

    Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with T-Sapphire marker

    Depositor

    Alejo Rodriguez-Fraticelli

    Article

    Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nsp2Cas9_AAV

    Plasmid

    #192143

    Purpose

    Expresses Nsp2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

    Insert

    Nsp2Cas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pG-HIV-LRT

    Plasmid

    #104592

    Purpose

    Standard for qPCR

    Depositor

    Kristine Yoder

    Article

    Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

    Insert

    HIV-1 late reverse transcripts
    

    Use

    Cloning vector

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Myo 1b-myc

    Plasmid

    #135064

    Purpose

    Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

    Depositor

    Martin Bähler

    Article

    Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

    Insert

    Myo 1b with 5' UTR (Myo1b Rat)
    

    Tags

    Linker and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSA110

    Plasmid

    #59784

    Purpose

    drives mCherry in C. elegans intestinal cells

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    elt-2 promoter (elt-2 Nematode)
    mCherry (worm)
    unc-54 3' UTR
    

    Expression

    Bacterial

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. p663-UBC-miniTurbo-V5-CDK13_IDG-K

    Plasmid

    #135266

    Purpose

    Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK13 (CDK13 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF hPGK mc2 IRES blast

    Plasmid

    #206239

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAC-DsRed-QF2-hsp70

    Plasmid

    #104876

    Purpose

    General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYSD300

    Plasmid

    #212930

    Purpose

    Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    AGA1 (AGA1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Atg24 1B

    Plasmid

    #197046

    Purpose

    His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

    Depositor

    Michael Ragusa

    Article

    Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

    Insert

    Atg24
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Available Since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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