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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,957 results for: pET

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Showing: 6001 - 6020 of 9957 results

  1. pTE5629

    Plasmid
    #250517
    Purpose
    Cell-free translocation reporter; translocation of a nanobody; N-terminal YncJ signal peptide; C-terminal pep86 tag
    Depositor
    Tobias Erb
    Publication
    Meier et al bioRxiv 2025.12.09.688994
    Insert
    T7-pYncJss-nanobody-HiBiT
    Tags
    pep86
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250517-plasmid-map-sequence-id-499121

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  2. pTE5605

    Plasmid
    #250512
    Purpose
    Mutant of pSecYEG; impaired in translocation and insertion; cell-free expression of functional SecYEG
    Depositor
    Tobias Erb
    Publication
    Meier et al bioRxiv 2025.12.09.688994
    Insert
    T7-secY(P84L)EG
    Expression
    Bacterial
    Mutation
    P84L
    Promoter
    T7
    Available since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250512-plasmid-map-sequence-id-498896

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  3. pTE5608

    Plasmid
    #250514
    Purpose
    Cell-free expression of SecA motor protein for translocation through SecYEG
    Depositor
    Tobias Erb
    Publication
    Meier et al bioRxiv 2025.12.09.688994
    Insert
    T7-secA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250514-plasmid-map-sequence-id-498893

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  4. pTE5627

    Plasmid
    #250516
    Purpose
    Super-active translocator mutant of pSecYEG; cell-free expression of functional SecYEG
    Depositor
    Tobias Erb
    Publication
    Meier et al bioRxiv 2025.12.09.688994
    Insert
    T7-secY(I408G)EG
    Expression
    Bacterial
    Mutation
    I408G
    Promoter
    T7
    Available since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250516-plasmid-map-sequence-id-498862

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  5. pTE5639

    Plasmid
    #250519
    Purpose
    Superactive inserter mutant of pSecYEG; cell-free expression of functional SecYEG
    Depositor
    Tobias Erb
    Publication
    Meier et al bioRxiv 2025.12.09.688994
    Insert
    T7-secY(T166L)EG
    Expression
    Bacterial
    Mutation
    T166L
    Promoter
    T7
    Available since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250519-plasmid-map-sequence-id-498895

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  7. pJTB126

    Plasmid
    #248910
    Purpose
    expresses the yeast analog-sensitive Smk1-Q120A and Ssp2 fused to glutathione-S-transferase in bacteria
    Depositor
    Edward Winter
    Publication
    Rimal et al Mol Cell Biol. 2020 Jul 29;40(16). pii:
    Inserts
    SMK1 Mitogen-activated Protein Kinase -analog-sensitive form (SMK1 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes from S288C form)
    Ssp2 Activator of the Smk1 MAPK (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)
    Tags
    glutathione-S-transferase
    Expression
    Bacterial
    Mutation
    codon 120 has been changed from Q to A (Smk1-Q120…
    Promoter
    T7
    Available since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248910-plasmid-map-sequence-id-497716

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  8. pJTB115

    Plasmid
    #248909
    Purpose
    Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria
    Depositor
    Edward Winter
    Publication
    Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-
    Inserts
    SMK1 Mitogen-activated Protein Kinase (SMK1 Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes, Budding Yeast)
    Ssp2 (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)
    Tags
    glutathione-S-transferase
    Expression
    Bacterial
    Mutation
    NdeI and NcoI sites were removed for cloning purp…
    Promoter
    T7
    Available since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248909-plasmid-map-sequence-id-497710

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  9. pJTB72

    Plasmid
    #248908
    Purpose
    Expresses yeast Smk1in bacteria
    Depositor
    Edward Winter
    Publication
    Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-
    Insert
    SMK1 Mitogen-activated Protein Kinase (SMK1 Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes, Budding Yeast)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248908-plasmid-map-sequence-id-497707

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  14. TrxA-(A AND C) OR T-SpyTag

    Plasmid
    #246947
    Purpose
    Expresses multifunctional construct for (AND)OR-gated release of ThioredoxinA from materials in response to TEV OR (sortase eSrtA(2A9) AND HRV-3C) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246947-plasmid-map-sequence-id-494406

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  20. Neo-A OR T OR C-SpyTag

    Plasmid
    #246948
    Purpose
    Expresses multifunctional construct for OR OR-gated release of Neoleukin (Neo) from materials in response to sortase eSrtA(2A9) OR TEV OR HRV-3C treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246948-plasmid-map-sequence-id-489555

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Showing: 6001 - 6020 of 9957 results