We narrowed to 10,309 results for: yeast

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pfa6a mScarlet-FKBP::KANMX

Plasmid

#185905

Purpose

Plasmid for cloning mScarlet fused to FKBP into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-FKBP

Tags

mScarlet fused to FKBP

Expression

Yeast

Promoter

SP6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDuRCC-K

Plasmid

#81193

Purpose

Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below., Please view…

Promoter

ROX3 and SNP52p

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMTKM07

Plasmid

#233479

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pETCON-PTX-S1-220

Plasmid

#67747

Purpose

Pertussis Toxin Subunit 1 Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

PTxS1-220

Expression

Bacterial and Yeast

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AU1-mTOR-E2419K

Plasmid

#19994

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

mammalian target of rapamycin-E2419K (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

Changed Glutamine 2419 to Lysine

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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Gal4BD-CRY2(535)

Plasmid

#75369

Purpose

Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC

Plasmid

#135465

Purpose

Membrane-anchored TF used for yeast directed evolution of TEV protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16

Tags

BFP, flag

Expression

Yeast

Promoter

TDH3

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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LOV-Turbo-V5_pRS415

Plasmid

#199667

Purpose

expresses LOV-Turbo in the yeast cytosol

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Tags

V5

Expression

Yeast

Promoter

Gal1,10

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMTKM05

Plasmid

#233477

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-yomRuby3-SpHIS5

Plasmid

#204287

Purpose

For C-terminal tagging using yeast optimized mRuby3

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pFA6-yomRuby3-SpHIS5

Tags

yomRuby3

Expression

Yeast

Promoter

none

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDuRCC-N

Plasmid

#81194

Purpose

Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please view depositor comments below and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pME-puro-Venus-FLAG-CD59

Plasmid

#50377

Purpose

Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Article

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Insert

Venus

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pJZC588

Plasmid

#62315

Purpose

sgRNA with 2x MS2 (wt+f6) for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 (wt+f6) binding module

Expression

Yeast

Promoter

SNR52

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AU1-mTOR-L1460P

Plasmid

#19995

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

mammalian target of rapamycin-L1460P (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

Changed Leucine 1460 to Proline

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
BAS286 C terminal SF-GFP hygBR

Plasmid

#74081

Purpose

S.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

super-folder GFP

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCDFDuet-trAtCCD7-OsD27(pYL726 )

Plasmid

#178285

Purpose

Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

OsD27
trAtCCD7

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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BB1_12_pGAP

Plasmid

#98498

Purpose

BB1 with insert: promoter PGAP

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

GAP promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo-HA-hApg12

Plasmid

#22950

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

autophagy related genes 12 (ATG12 Human)

Tags

3xHA

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pJZC603

Plasmid

#62317

Purpose

sgRNA with 2x PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x PP7 RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pIMVAd40R

Plasmid

#98309

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

Insert

ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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