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pET21a Mo-iPGM-bio

Plasmid

#179614

Purpose

Expresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

M. orale iPGM bio-6His

Tags

His tag and biotinylation sequence

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Hp-iPGM-bio

Plasmid

#179615

Purpose

Expresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

H. pylori iPGM bio-6His

Tags

His tag and biotinylation sequence

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

Plasmid

#153445

Purpose

E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.XDocB

Tags

6xHis and ybbr tag

Expression

Bacterial

Mutation

Serine 199 was mutated to amber codon (TAG). Argi…

Promoter

T7 promoter

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

Plasmid

#153445

Purpose

E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.XDocB

Tags

6xHis and ybbr tag

Expression

Bacterial

Mutation

Serine 199 was mutated to amber codon (TAG). Argi…

Promoter

T7 promoter

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

Plasmid

#153444

Purpose

E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.XDocB

Tags

6xHis and ybbr tag

Expression

Bacterial

Mutation

Serine 199 was mutated to amber codon (TAG). Argi…

Promoter

T7 promoter

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

Plasmid

#153444

Purpose

E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.XDocB

Tags

6xHis and ybbr tag

Expression

Bacterial

Mutation

Serine 199 was mutated to amber codon (TAG). Argi…

Promoter

T7 promoter

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-F241S

Plasmid

#140740

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation F241S and GFP as two separate proteins

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-T2A-PTENF241S (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

F241S

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-F241S

Plasmid

#140740

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation F241S and GFP as two separate proteins

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-T2A-PTENF241S (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

F241S

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-W274L

Plasmid

#140742

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation W274L and GFP as two separate proteins

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-T2A-PTENW274L (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

W274L

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-W274L

Plasmid

#140742

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation W274L and GFP as two separate proteins

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-T2A-PTENW274L (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

W274L

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-D252G

Plasmid

#140741

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation D252G and GFP as two separate proteins

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-T2A-PTEND252G (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

D252G

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-D252G

Plasmid

#140741

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation D252G and GFP as two separate proteins

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-T2A-PTEND252G (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

D252G

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG3_CH2charge

Plasmid

#105858

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG3 heavy chain (Ighg3 Mouse)

Expression

Mammalian

Mutation

mutated heavy chain of mouse IgG3, muatations: Hi…

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG3_CH2charge

Plasmid

#105858

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG3 heavy chain (Ighg3 Mouse)

Expression

Mammalian

Mutation

mutated heavy chain of mouse IgG3, muatations: Hi…

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_LC_R191K_R254K

Plasmid

#104466

Purpose

express MBP hnRNPA2 LC with 2 R to K mutations

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

R191K R254K

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_LC_R191K_R254K

Plasmid

#104466

Purpose

express MBP hnRNPA2 LC with 2 R to K mutations

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

R191K R254K

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.27

Plasmid

#99362

Purpose

Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Glutamic acid 129 to Lysine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.27

Plasmid

#99362

Purpose

Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Glutamic acid 129 to Lysine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

Plasmid

#92315

Purpose

Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

B1 domain of protein L (PL) mutant K16TAG

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70b (p70a promoter with one mutation)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

Plasmid

#92315

Purpose

Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

B1 domain of protein L (PL) mutant K16TAG

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70b (p70a promoter with one mutation)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge