Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,719 results for: kit

Clear keywords
Showing: 6041 - 6060 of 6719 results

  1. ARB367

    Plasmid
    #124050
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124050-plasmid-map-sequence-id-252515

    Enlarge

  2. pVL43 - pNST3>>

    Plasmid
    #116009
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Thomas Greb
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    116009-plasmid-map-sequence-id-227979

    Enlarge

  3. pVL42 - pLTP1>>

    Plasmid
    #116008
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Thomas Greb
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pLTP1:GR-LhG4:tLT1:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    116008-plasmid-map-sequence-id-227906

    Enlarge

  11. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163094-plasmid-map-sequence-id-320582

    Enlarge

  13. p426_Cas9_gRNA-ARS1014a

    Plasmid
    #87396
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87396-plasmid-map-sequence-id-193439

    Enlarge

  14. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

    Enlarge

  17. p426_Cas9_gRNA-ARS106a

    Plasmid
    #87382
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS106a sequence ATACGGTCAGGGTAGCGCCC in yeast chromosome 1.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS106a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87382-plasmid-map-sequence-id-193424

    Enlarge

  18. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid
    #55201
    Purpose
    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55201-plasmid-map-sequence-id-240663

    Enlarge

  19. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50587-plasmid-map-sequence-id-75659

    Enlarge

  20. pOD2044-intDEG

    Plasmid
    #89366
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Promoter
    Pelt-2
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89366-plasmid-map-sequence-id-177231

    Enlarge

Showing: 6041 - 6060 of 6719 results