We narrowed to 25,266 results for: multi

1. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

   Plasmid

   #20943

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available Since

   July 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. mRFP-FKBP12

   Plasmid

   #67514

   Purpose

   FKBP12 only

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

   Insert

   FKBP12 (FKBP1A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRGE32

   Plasmid

   #63159

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

   Plasmid

   #59791

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized VP64 dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Available Since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42337

   Purpose

   Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY026

   Plasmid

   #84741

   Purpose

   Expresses huAsCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huAsCpf1
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2 GFP-GSK3-MAPK

   Plasmid

   #29689

   Purpose

   GFP reporter protein fused to three GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site primed by MAPK
   

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site

   Promoter

   SP6

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA PKB T308D S473D pcDNA3

   Plasmid

   #14751

   Depositor

   Jim Woodgett

   Article

   Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60

   Insert

   PKB DD (AKT1 Bovine)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T308D S473D. Constitutively active mutant.

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBS-MXT GIRK2

   Plasmid

   #20675

   Depositor

   Henry Lester

   Article

   Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

   Insert

   Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
   

   Use

   Xenopus expression

   Tags

   3' UTR Xenopus B- globin and 5' UTR Xen…

   Mutation

   A369V

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-NR1H2

    Plasmid

    #79513

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    LXR BETA (NR1H2 Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1 puro shRNA E-cadherin

    Plasmid

    #18801

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin shRNA (CDH1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. STAGR_gRNAScaffold_mU6

    Plasmid

    #102844

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert gRNAScaffold_mU6
    

    Use

    Pcr template for stagr inserts

    Promoter

    mU6

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Thy1 promoter construct

    Plasmid

    #20736

    Depositor

    Joshua Sanes

    Article

    Feng et al Neuron. 2000 Oct . 28(1):41-51.

    Insert

    Thy1 promoter (Thy1 Mouse)
    

    Expression

    Mammalian

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. -2.5Kb MMP10

    Plasmid

    #53973

    Purpose

    Luciferase reporter for mouse MMP10 2.5kb promoter

    Depositor

    Michael Chin

    Article

    Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

    Insert

    mouse MMP10 2.5Kb promoter (Mmp10 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PCR from C57BL6/J

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY027

    Plasmid

    #84742

    Purpose

    Expresses huLbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huLbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ133B2.0

    Plasmid

    #99892

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. STAGR_gRNAScaffold_hH1

    Plasmid

    #102841

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert gRNAScaffold_hH1
    

    Use

    Pcr template for stagr inserts

    Promoter

    hH1

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2 GFP-GSK3mut-MAPK

    Plasmid

    #29690

    Purpose

    GFP reporter protein fused to three mutated GSK3 phosphorylation sites

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    

    Tags

    Flag and Flag-EGFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site, GSK3 …

    Promoter

    SP6

    Available Since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3E-2xFYVE_hrs

    Plasmid

    #67676

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    2xFYVEhrs
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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