We narrowed to 59,538 results for: cloning

1. pFOSGE_COF4A02

   Plasmid

   #71021

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Ube3a (Ube3a Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

2. p663-UBC-miniTurbo-V5-STK3_IDG-K

   Plasmid

   #189879

   Purpose

   Gateway destination clone of STK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   STK3 (STK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p663-UBC-miniTurbo-V5-MARK3_IDG-K

   Plasmid

   #170583

   Purpose

   Gateway destination clone of MARK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-MARK3 (MARK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRL2960

   Plasmid

   #70694

   Purpose

   pDU1-E. coli shuttle vector (Km-r) with extended polylinker

   Depositor

   Peter Wolk

   Article

   Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

   Type

   Empty backbone

   Use

   Bacterial shuttle vector

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p667-UBC-MAP3K14-V5-miniTurbo_IDG-K

   Plasmid

   #189903

   Purpose

   Gateway destination clone of MAP3K14 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   MAP3K14 (MAP3K14 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICH87633

   Plasmid

   #50271

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pICSL70002

   Plasmid

   #50333

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pF CAG mc2 IRES hygro

   Plasmid

   #206234

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC-Ura-Cen-lvl1

   Plasmid

   #176182

   Purpose

   preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC-X2

    Plasmid

    #176152

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMC-X3

    Plasmid

    #176153

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC-XI1

    Plasmid

    #176155

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMC-XI5

    Plasmid

    #176158

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMC-XII2

    Plasmid

    #176160

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMINTC3GH (Apr)

    Plasmid

    #110078

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GGE020

    Plasmid

    #120777

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T1-3_Lip2
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICH51277

    Plasmid

    #50268

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid

    #168207

    Purpose

    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

    Depositor

    Markus Hafner

    Article

    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

    Type

    Empty backbone

    Use

    Unspecified; Pdest gateway

    Tags

    V5 APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMEXC3GH (Apr)

    Plasmid

    #110083

    Purpose

    Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p663-UBC-miniTurbo-V5-DSTYK _IDG-K

    Plasmid

    #158182

    Purpose

    Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-DSTYK (DSTYK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge