We narrowed to 20,079 results for: INO

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pAAV-CMV-dSa VP64 Hbb

Plasmid

#99693

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)

Use

AAV and CRISPR

Tags

VP64

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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AA181

Plasmid

#216024

Purpose

Fragmid fragment: (Cas protein) unmodified AsCas12a; not preferred

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas12a_v1.1 [As]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA048

Plasmid

#216016

Purpose

Fragmid fragment: (Cas protein) deactivated Cas

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

dCas12a (D908A)_v1.1 [EnAs]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_816

Plasmid

#216098

Purpose

CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_05-mEGFP_WRPE-SV40

Plasmid

#194689

Purpose

hSyn1 driven expression of the calcium recorder Caprola_05 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_04

Plasmid

#194665

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_04 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_06

Plasmid

#194667

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_06 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_07

Plasmid

#194668

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_07 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_07

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_08

Plasmid

#194669

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_08 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_08

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_10

Plasmid

#194671

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_10 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_10

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_11

Plasmid

#194672

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_11 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_11

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_12

Plasmid

#194673

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_12 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_12

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_02

Plasmid

#194663

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_02 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_02

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_03

Plasmid

#194664

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_03 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_03

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC7-20XUAS-IVS-NES_Caprola5-mEGFP

Plasmid

#205704

Purpose

Gal4 driven expression of EGFP-Caprola5 for targeted expression in Drosophila flies

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola5-EGFP

Expression

Insect

Promoter

20 Gal4

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mScarlet-I-eDHFR-ORP5(ΔPH)

Plasmid

#214277

Purpose

Mammalian expression of ORP5(ΔPH) fused to mScarlet-I-tagged E. coli DHFR

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mScarlet-I-eDHFR-ORP5(253-879) (OSBPL5 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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TLNRD1_35-345_eGFP

Plasmid

#208881

Purpose

Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 (TLNRD1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acid sequence 35-345

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TLNRD1_5H_eGFP

Plasmid

#208861

Purpose

Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 5 helix bundle (TLNRD1 Human)

Expression

Mammalian

Mutation

Deleted amino acid 142-273

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-hSynapsin-Nluc-EPAC-VV

Plasmid

#204846

Purpose

Produce lentivirus encoding Nluc-EPAC sensor in neuronal cell type

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

Nluc-EPAC sensor

Use

Lentiviral

Available Since

Aug. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-SVA-lncRNA-AK057321-mcherry

Plasmid

#202813

Purpose

Lentiviral vector to express of SVA-lncRNA AK057321 with CMV for mcherry expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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