We narrowed to 18,581 results for: MUT

1. HA-HIF2α MT-pBabe-Hygro

   Plasmid

   #22134

   Depositor

   William Kaelin

   Article

   Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA "silent"("conservative"…

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI.neo-rPAM1-M314I

   Plasmid

   #145802

   Purpose

   expresses catalytically inactive rat PAM-1 in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

   Insert

   rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
   

   Expression

   Mammalian

   Mutation

   changed methionine 314 to isoleucine to inactivat…

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-mCherry-TSC2(AA)

   Plasmid

   #192442

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A) (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMyc1ElbLuc,

   Plasmid

   #53243

   Purpose

   reporter plasmid containing 1 myc cDNA binding sites

   Depositor

   Roger Davis

   Article

   Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

   Insert

   pMyc1ElbLuc (MYC Human)
   

   Mutation

   To prepare plasmids with one, two, three, and fou…

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pDEST8_FLAGHA_FUS_HIS

   Plasmid

   #26375

   Depositor

   Thomas Tuschl

   Article

   Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

   Insert

   FUS (FUS Human)
   

   Tags

   FLAG/HA and His

   Expression

   Insect

   Mutation

   delta 527 (STOP codon)

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

6. C1-FKBP(F36V):mCherry-Akt2(K14A)

   Plasmid

   #72906

   Purpose

   Recruitable Akt2(K14A) mutant

   Depositor

   Ivan Yudushkin

   Article

   Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

   Insert

   RAC-beta serine/threonine-protein kinase (AKT2 Human)
   

   Tags

   FKBP(F36V), mCherry

   Expression

   Mammalian

   Mutation

   K14A mutation

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWZL Blast H-Ras G12V T35S

   Plasmid

   #12278

   Depositor

   Bob Weinberg

   Article

   Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

   Insert

   H-Ras G12V T35S (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Contains G12V activating mutation. The T35S mutat…

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pGST TCF4 DBD

   Plasmid

   #16488

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   TCF4 DNA binding domain (TCF7L2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 265-496

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pCR8/GW/TOPO-SRF

   Plasmid

   #98618

   Purpose

   Gateway entry TOPO TA cloning vector for SRF

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   SRF (Srf Mouse)
   

   Use

   Topo ta cloning vector

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-mNRDc-E>A-V5

    Plasmid

    #51298

    Purpose

    expression of the inactive mutant of mouse NRDc in mammalian cells

    Depositor

    Eiichiro Nishi

    Article

    Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

    Insert

    NRDc E247A (Nrd1 Mouse)
    

    Tags

    HIS and V5

    Expression

    Mammalian

    Mutation

    E247A, enzymatic inactive mutant

    Promoter

    CMV

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

11. PIK3CA - Exon9 E545K (pFastBac)

    Plasmid

    #16640

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Baculoviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    E545K

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pAFMW-Nbr-D435A/E437A

    Plasmid

    #40829

    Purpose

    Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

    Depositor

    Phillip Zamore

    Article

    Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

    Insert

    Nbr D435A/E437A (Nbr Fly)
    

    Tags

    3xFLAG and 6xMyc

    Expression

    Insect

    Mutation

    D435A/E437A, part of the DEDD motif; mutations p…

    Promoter

    Actin5C

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP-RapR-FAK-YM-Y397F

    Plasmid

    #25930

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse, Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUAST-SIK3 T196E

    Plasmid

    #52975

    Purpose

    insect expression of SIK3 T196E constitutively active mutant

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    T196E constitutively active mutant

    Promoter

    Hsp70

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

15. p4979 pcDNA4-E6AP III L502P

    Plasmid

    #32892

    Depositor

    Peter Howley

    Article

    Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

    Insert

    E6AP (UBE3A Human)
    

    Tags

    6xHis Express and T7-tag

    Expression

    Mammalian

    Mutation

    L502P

    Promoter

    CMV

    Available Since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

16. p4978 pcDNA4-E6AP III E550L

    Plasmid

    #32891

    Depositor

    Peter Howley

    Article

    Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

    Insert

    E6AP (UBE3A Human)
    

    Tags

    6xHis Express and T7-tag

    Expression

    Mammalian

    Mutation

    E550L

    Promoter

    CMV

    Available Since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCEP4-gp120(BaL)-CD4

    Plasmid

    #98957

    Purpose

    Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    HIV-1 (BaL) gp120
    

    Tags

    CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

19. BtGRK2-sYFP2

    Plasmid

    #137778

    Purpose

    Visualization of GRK2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Bovine GRK2 (GRK2 Bovine)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE-eGFP-F1A

    Plasmid

    #211458

    Purpose

    Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation mutant control peptide (F1A)

    Depositor

    Linda DeGraffenried

    Article

    McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

    Insert

    Flotillin-1-palmitoylation mutant control peptide (FLOT1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    C34A

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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