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Human Brunello kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

Pooled Library

#75314

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 1-4 in lentiGuide-Puro backbone

Pooled Library

#75312

Purpose

Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 5-8 in lentiCRISPRv2 backbone

Pooled Library

#75315

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 5-8 in lentiGuide-Puro backbone

Pooled Library

#75313

Purpose

Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-Axin-IRES-GFP

Plasmid

#14716

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

Axin (Axin1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
Sufu_pLENTI-CAG-IRES-GFP

Plasmid

#176993

Purpose

Mammalian lentiviral expression vector encoding Sufu

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

Sufu (Sufu Mouse)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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Human sgRNA library Brunello in lentiGuide-Puro

Pooled Library

#73178

Purpose

Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
Mouse sgRNA library Brie in lentiGuide-Puro

Pooled Library

#73633

Purpose

Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
MYR-FR-C2D-EGFR

Plasmid

#179262

Purpose

Light-induced clustering of EGFR cytosolic domains

Depositor

Jared Toettcher

Article

Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Cry2 PHR, FUS, FusionRed, and Myristoylation sequ…

Promoter

SFFV

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-TBX20

Plasmid

#170705

Purpose

doxycycline-inducible overexpression of TBX20

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

TBX20 (TBX20 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-PBX1

Plasmid

#170699

Purpose

doxycycline-inducible overexpression of PBX1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

PBX1 (PBX1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-MYOCD

Plasmid

#170696

Purpose

doxycycline-inducible overexpression of MYOCD

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MYOCD (MYOCD Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-SMAD6

Plasmid

#170700

Purpose

doxycycline-inducible overexpression of SMAD6

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SMAD6 (SMAD6 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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FGFR1-optoDroplet

Plasmid

#111509

Purpose

For optogenetic clustering of FGFR1

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

FGFR1 (FGFR1 Human)

Use

Lentiviral

Tags

Cry2, FUS, Fusion Red, and Myristoylation sequenc…

Promoter

SFFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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tetO-TCF21

Plasmid

#170706

Purpose

doxycycline-inducible overexpression of TCF21

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

TCF21 (TCF21 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-SMARCD3

Plasmid

#170701

Purpose

doxycycline-inducible overexpression of SMARCD3

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SMARCD3 (SMARCD3 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-SNAI2

Plasmid

#170702

Purpose

doxycycline-inducible overexpression of SNAI2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SNAI2 (SNAI2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFETCH_ATF1

Plasmid

#64689

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human ATF1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ATF1 Homology Arms (ATF1 Human)

Tags

3X Flag P2A Neo

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_Vidr_007

Plasmid

#244774

Purpose

Express mEGFP-tagged intrinsically disordered protein (IDR), Vidr_007, derived from human protein FUS

Depositor

Richard Kriwacki

Article

Maiti et al Sci Adv. 2025 Dec 5;11(49):eady1420. doi

Insert

Vidr_007

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Unmutated

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

Plasmid

#209082

Purpose

NFIB and SOX9 mediated astrocye induction

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
mKate2

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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