We narrowed to 94,543 results for: sap

1. PRKCG_Halo_C_allele

   Plasmid

   #178146

   Purpose

   Donor vector for endogenous tagging of human PRKCG at the C-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (PRKCG Human)
   

   Use

   Donor vector

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDX4-donor

   Plasmid

   #176343

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   CDX4 (CDX4 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAZF1-donor

   Plasmid

   #176352

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   JAZF1 (JAZF1 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SspB-HaloTag-DHPH1-TRIO

   Plasmid

   #176115

   Purpose

   Heterodimerization with iLID, Rac1 and RhoG activation

   Depositor

   Dorus Gadella

   Insert

   TRIO (TRIO Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEntr1a-SspB-HaloTag-TRIO-DHPH1

   Plasmid

   #176133

   Purpose

   Heterodimerization with iLID, Rac1 and RhoG activation

   Depositor

   Dorus Gadella

   Insert

   TRIO (TRIO Human)
   

   Use

   Entry vector

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB2HoptX

   Plasmid

   #163832

   Purpose

   Inactive two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B

   Depositor

   Jerome Fox

   Insert

   SpecR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   MidT Substrate Y4F

   Promoter

   Pro1;ProD;pLacZ-Opt

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. WDR55

   Plasmid

   #167844

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   WDR55 (WDR55 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   April 1, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. C-BRPF3-8

   Plasmid

   #166516

   Purpose

   scFv of a human scaffold targeting Bromodomain and PHD finger-containing protein 3. Antigen coverage aa 1056-1195 of 1205, PWWP domain.

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6WAT

   Plasmid

   #162268

   Purpose

   Baculovirus expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   PHF1 (PHF1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. 6PED

    Plasmid

    #162265

    Purpose

    Baculovirus expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    HEMK2 (N6AMT1 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. 6VYC

    Plasmid

    #162266

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR91 (WDR91 Human)
    

    Use

    Baculovirus expresssion

    Tags

    6xHis-TEV

    Promoter

    Polyhedrin

    Available Since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET11b-MIF H62F

    Plasmid

    #149445

    Purpose

    Expression of MIF H62F in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    H62F

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11b-MIF H62A

    Plasmid

    #149443

    Purpose

    Expression of MIF H62A in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    H62A

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET11b-MIF H62Y

    Plasmid

    #149442

    Purpose

    Expression of MIF H62Y in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    H62Y

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET11b-MIF Y99G

    Plasmid

    #149441

    Purpose

    Expression of MIF Y99G in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    Y99G

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV-gRNA-BTRC-MGMT_2

    Plasmid

    #136416

    Purpose

    Lentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.

    Depositor

    Massimo Squatrito

    Insert

    U6_sgRNA(BTRC)_U6_sgRNA(MGMT)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTP63.1.0-gDNA

    Plasmid

    #132432

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TP63 (TP63 Human)
    

    Use

    CRISPR

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TBXTA-c025

    Plasmid

    #139762

    Purpose

    Protein expression/GST pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, Full-length, G177D (TBXT Human)
    

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids S2- M435

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TBXTA-c023

    Plasmid

    #139760

    Purpose

    Protein expression/GST pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, WT (TBXT Human)
    

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    Contains only amino acids E41-D225

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TBXTA-c022

    Plasmid

    #139759

    Purpose

    Protein expression/crystallography

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, G177D (TBXT Human)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids E41-A214

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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