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(363) pcDNA3.1-Nup62-Ubc-Flag-EPEA

Plasmid

#168098

Purpose

Nucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tag

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

Nup62-Ubc-Flag-EPEA

Tags

EPEA-tag, Flag-tag, and Ubc-Tag

Expression

Mammalian

Mutation

P229A, S283T on Nup62 native sequence

Promoter

T7/cmv

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

Plasmid

#133790

Purpose

CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized NmeCas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

Plasmid

#105803

Purpose

Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: 3C-FLAG; ORF2: TEV-mClover3

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag Jip3b(T266A, T276A, T287A)

Plasmid

#58924

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed T266A, T276A, T287A

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

Plasmid

#113162

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Tags

FLAG

Expression

Mammalian

Mutation

D10A

Promoter

CAG

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG mTOR 1968-2549 S2035T

Plasmid

#23000

Depositor

Jing Huang

Article

Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

mTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

S2035T aa 1968-2549 only

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-myc NES-mCE NLS-Flag

Plasmid

#82468

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-NES-mCE-Flag (Rngtt Mouse, HIV)

Tags

Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-Venus-FLAG-LINXa4-Bre1

Plasmid

#73618

Purpose

Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

Venus

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A

Promoter

ADH1

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D

Plasmid

#32460

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

S129D, S133D, S137D

Promoter

human PGK

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)

Plasmid

#47430

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK3b(Glu, delete LXL)

Plasmid

#50450

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted L25-I27, changed Serine 218 to Glutamic A…

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

Plasmid

#90011

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C S216A
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Ser 216 to Ala

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRDAX-018 FLAG Venus210[1] IRES Venus210[2]

Plasmid

#130128

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L57E/L113E/W284A

Plasmid

#47343

Purpose

triple mutation used for co-IP

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L57E/L113E/W284A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Tagged Clavesin2 (pCMV-Tag2B-Sec14b)

Plasmid

#47431

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin2 (CLVS2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

Plasmid

#169194

Purpose

Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells

Depositor

John Diffley

Article

Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

Insert

3xFlag-His6-PLpro (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-His6

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 494-1216 (USP)

Plasmid

#78588

Purpose

Expression flag-USP19 494-1216 (USP) in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1216 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1216 residue…

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN beta delta C term

Plasmid

#40931

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

C terminus deleted

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only