We narrowed to 11,952 results for: GFP expression plasmids

1. pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

   Plasmid

   #175554

   Purpose

   Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   NANOG
   

   Use

   Mouse Targeting

   Tags

   FKBPV-HA2-P2A-eGFP

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

   Plasmid

   #200634

   Purpose

   Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   PTEN (PTEN Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRM210 LGP (Luciferase GFP Puro)

   Plasmid

   #174722

   Purpose

   Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   eGFP
   pac
   

   Use

   Lentiviral and Luciferase

   Tags

   E2A linker and F2A linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GC52 pEGFPN1 anti-Gephyrin nanobody

   Plasmid

   #135224

   Purpose

   Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
   

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-UBC-hCD59-T2A-GFP

   Plasmid

   #205460

   Purpose

   lentiviral plasmid for expression of human CD59

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD59 (CD59 Human)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. IC65 pEGFPN1 anti-IRSp53 nanobody

   Plasmid

   #135227

   Purpose

   Mammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))
   

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn-HA-PGRN-LAMP1TM-IRES-GFP

   Plasmid

   #234877

   Purpose

   Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.

   Depositor

   Andrew Arrant

   Article

   Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

   Insert

   Progranulin (GRN Human)
   

   Use

   Lentiviral

   Tags

   HA tag and LAMP-1 transmembrane domain and cytoso…

   Expression

   Mammalian

   Promoter

   hSYn

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tet-O-FUW-EGFP-P2A-mCherry

   Plasmid

   #203868

   Purpose

   Tet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   EGFP
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AC50 pEGFPN1 anti-AMIGO-1 nanobody

    Plasmid

    #135225

    Purpose

    Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-AMIGO-1 (Mouse) recombinant llama nanobody AC50 (Amigo1 L. glama (llama))
    

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HT107_pAAV_hSyn-DiO-SomQuasAr6a_EGFP-P2A-somCheRiff_HA

    Plasmid

    #178826

    Purpose

    Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    SomQuasAr6a_EGFP-P2A-somCheRiff_HA
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP; HA

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SS80 pEGFPN1 anti-SAPAP2 nanobody

    Plasmid

    #135228

    Purpose

    Mammalian expression of anti-SAPAP (Rat) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-SAPAP2 (Rat) recombinant llama nanobody SS80 (Dlgap2 L. glama (llama))
    

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-FDIO-ArchT-GFP

    Plasmid

    #124640

    Purpose

    AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    ArchT
    

    Use

    AAV; Flp/frt

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-TagRFP658-P2A-GFP

    Plasmid

    #178971

    Purpose

    Expresses TagRFP658 and GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    TagRFP658-P2A-GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AC27 pEGFPN1 anti-AMIGO-1 nanobody

    Plasmid

    #135226

    Purpose

    Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-AMIGO-1 (Mouse) recombinant llama nanobody AC27 (Amigo1 L. glama (llama))
    

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

    Plasmid

    #178827

    Purpose

    Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    SomQuasAr6a_EGFP-P2A-somCheRiff_HA
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP, HA

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. BE3(E63Q)-P2A-EGFP (pRZ189)

    Plasmid

    #123613

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(E63Q)-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    E63Q in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]

    Plasmid

    #153532

    Purpose

    AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon-GFP
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Promoter

    EF1α1.1

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG coca-5HT3-IRES-GFP

    Plasmid

    #242193

    Purpose

    Expression vector for cocaine-gated chemogenetic cation channel

    Depositor

    Scott Sternson

    Article

    Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

    Insert

    coca-5HT3-IRES-GFP (Htr3a Mouse)
    

    Expression

    Mammalian

    Mutation

    L141G G175K Y210F Y217F

    Promoter

    CAG

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

    Plasmid

    #199349

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOEu

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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