We narrowed to 13,172 results for: GFP expression plasmids

1. AAV-EGFP-APOBEC1-YTHmut

   Plasmid

   #209320

   Purpose

   AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTHmut cassette.

   Depositor

   Magdalena Koziol

   Article

   Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

   Insert

   APOBEC1-YTHmut-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   YTH domain lacks AA 385-409 comprising the m6A-bi…

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138132

   Purpose

   Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   EGFP-anti-mouse-IgG(kappa)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

   Plasmid

   #114378

   Purpose

   expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-KA2N-EGFP (NEWENTRY )
   

   Use

   AAV

   Tags

   EGFP and KA2N

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)

   Plasmid

   #126025

   Purpose

   express RFP- and mCer-tagged LARIAT for optogenetic clustering

   Depositor

   Eurico Morais-de-Sá

   Article

   Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

   Insert

   CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)
   

   Use

   Tags

   RFP

   Expression

   Insect

   Mutation

   WT

   Promoter

   Hsp70

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

   Plasmid

   #208292

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA inlaid into nSpCas9(D10A)

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP

   Plasmid

   #176282

   Purpose

   Viral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   TeNT-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin I

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAGGS-Flex/3'USS(Luc)-GFP

   Plasmid

   #197887

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49228

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-short

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQLL-MAG[EF1α-copGFP]

   Plasmid

   #218324

   Purpose

   Donor plasmid of MAG Transposon with copGFP mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   copGFP mammalian expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMV-SB100X[EF1α-copGFP]

    Plasmid

    #218323

    Purpose

    Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-Chronos-GFP

    Plasmid

    #99232

    Purpose

    AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn-Chronos-GFP

    Plasmid

    #59170

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKII-Chrimson-GFP

    Plasmid

    #62718

    Purpose

    AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chrimson-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1α1.1-Chronos-GFP

    Plasmid

    #122098

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOxyR_gfp

    Plasmid

    #194154

    Purpose

    Green Fluorescent Reporter of intracellular hydrogen peroxide

    Depositor

    Sven Panke

    Article

    Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

    Insert

    transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PoxyR, PoxyS

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid

    #129025

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid

    #129026

    Purpose

    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TLCV2-Scal-T2A-EGFP-Puro

    Plasmid

    #234949

    Purpose

    Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. TLCV2-ApaLI-T2A-EGFP-Puro

    Plasmid

    #234948

    Purpose

    Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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