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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,837 results for: pET

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Showing: 6161 - 6180 of 9837 results

  1. GB1-PRD(703-868, P801G, C813S, S863C)-Strep

    Plasmid
    #164447
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Cysteine 813 to Serine, S…
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164447-plasmid-map-sequence-id-324034

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  3. pBtPIPLC_N168C His

    Plasmid
    #173809
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Anne Gershenson, Mary F Roberts
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    Asn168Cys for labeling
    Promoter
    T7
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173809-plasmid-map-sequence-id-341161

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  4. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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  19. KSP_HNL

    Plasmid
    #129772
    Purpose
    Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    129772-plasmid-map-sequence-id-264857

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  20. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    Unc104 (unc-104 Nematode)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129773-plasmid-map-sequence-id-263568

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Showing: 6161 - 6180 of 9837 results