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pDM1049

Plasmid

#108992

Purpose

Inducible expression vector (dox-on), C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

GFP

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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OptoOFF-MxCBD-mFGFR1_623

Plasmid

#105825

Purpose

Expresses OptoOFF-MxCBD-mFGFR1 (light-inactivated receptor tyrosine kinase)

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Insert

OptoOFF-MxCBD-mFGFR1

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM-gate

Plasmid

#113758

Purpose

Gateway destination vector only containing the Gateway cassette and pGEM-T Easy backbone. This can be used with Gateway-compatible Homology-Directed Repair.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

gateway cassette

Use

Gateway destination vector

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBW102ParsR-Amp32C

Plasmid

#78637

Purpose

pBW100ParsR carrying 30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBW820_pCAG-pExpr-FULL-ADDER

Plasmid

#87552

Purpose

Encodes full adder logic in response to Cre, Flp and VCre recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVf-EnhCB-lacZnls

Plasmid

#35642

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Type

Empty backbone

Use

AAV

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pCfB6577

Plasmid

#106129

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A3012

Plasmid

#91045

Purpose

Module A, Promoter: PvUbi1, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

PvUbi1

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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5n1 (560)

Plasmid

#70730

Purpose

TetR/PhlF/SrpR/LambdaCI/LacI BCD7 5-node v41-2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Inserts

srpR-ssrA
lambdaCI-ssrA
phlF-ssrA
lacI-ssrA
tetR-ssrA

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTRANS_110

Plasmid

#91202

Purpose

Transformation, Type: non-T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pBsaHFm

Plasmid

#85103

Purpose

Heme-FMN domain of P450 BM3 variant (A74G)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Heme-FMN domain of P450 BM3

Use

Cloning vector

Mutation

A74G

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB5219

Plasmid

#106135

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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tdEos-Paxillin-KM-C-10

Plasmid

#57654

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin KM

Tags

tdEos

Expression

Mammalian

Mutation

Mutation to remove Kozak sequence

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLITMUS-rpoN-cI5G6G-J23106-geneIII

Plasmid

#80906

Purpose

Phagemid (encodes cI5G6G and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6G

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6T-J23106-geneIII

Plasmid

#80907

Purpose

Phagemid (encodes cI5G6T and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6T

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5T6T-J23106-geneIII

Plasmid

#80908

Purpose

Phagemid (encodes cI4A5T6T and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5T6T

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ159

Plasmid

#69308

Purpose

Gateway entry vector with hSpCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9D10A (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-3HA

Plasmid

#17373

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3HA (WNT3 Human, Mouse)

Tags

HA

Expression

Bacterial

Mutation

This gene is a fusion of the murine and human wnt…

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pMOD_A3010

Plasmid

#91044

Purpose

Module A, Promoter: ZmUbi, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAW-GFP-2xmiR-34 (pAW-AS-25)

Plasmid

#40833

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Two target sites perfectly complementary to miR-34

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only