We narrowed to 8,829 results for: sgRNA

1. pCB-CRISPRi

   Plasmid

   #221136

   Purpose

   Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   3xF-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial

   Promoter

   cbu1169 promoter

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p8390 LentiCRISPRv2 Neo sgNT-2

   Plasmid

   #221650

   Purpose

   Expression of non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

   Insert

   non-targeting sgNT-2
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GEARBOCS-Vamp2-GeneTRAP

   Plasmid

   #218187

   Purpose

   To KO and genetrap mouse Vamp2 simultaneously

   Depositor

   Cagla Eroglu

   Article

   Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

   Insert

   sgRNA (Vamp2 Mouse)
   

   Use

   AAV and CRISPR

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GEARBOCS-Vamp2-Tag-HA

   Plasmid

   #218188

   Purpose

   To tag endogenous mouse VAMP2 with HA

   Depositor

   Cagla Eroglu

   Article

   Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

   Insert

   sgRNA (Vamp2 Mouse)
   

   Use

   AAV and CRISPR

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV/CMV-SCLM

   Plasmid

   #216758

   Purpose

   AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon

   Depositor

   George Augustine

   Insert

   SuperClomeleon followed by WPRE and human growth hormone polyA terminator
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   CMV (cytomegalovirus)

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210749

   Purpose

   Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   CjSp D8A Cas9
   Bilbo sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D8A, N-terminal Cj Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUCSh2-mRFP

   Plasmid

   #170982

   Purpose

   Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   mRFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCZGY2748

   Plasmid

   #193331

   Purpose

   Site specific CRISPR/Cas9 editing of C. elegans Chr I

   Depositor

   Yishi Jin

   Article

   Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

   Insert

   sgRNA for ttTi4348
   

   Tags

   None

   Expression

   Worm

   Promoter

   U6

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pICSL1P4_AtU626Ter26

   Plasmid

   #191772

   Purpose

   Cas9 guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   SpCas9 guide scaffold with AtU626 promoter/terminator
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICSL1P7_AtU626Ter26

    Plasmid

    #191775

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICSL1P2_AtU626Ter26

    Plasmid

    #191770

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL1P5_AtU626Ter26

    Plasmid

    #191773

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICSL1P6_AtU626Ter26

    Plasmid

    #191774

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSB700 lenti gRNA Hygro_zhang2.0

    Plasmid

    #167915

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSB700 lenti gRNA EYFP_zhang2.0

    Plasmid

    #167914

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTJV1ScGm-rpoB

    Plasmid

    #166979

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    gentamycin-3-acetyltransferase (aac(3)-Ia )
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPR v2-sgSmg7

    Plasmid

    #161809

    Purpose

    guide RNA for Smg7

    Depositor

    Yi Zhang

    Article

    Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

    Insert

    sgRNA targeting mouse Smg7 (Smg7 Mouse, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PTG

    Plasmid

    #127198

    Purpose

    Single template U6 promoter minimal size plasmid

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Type

    Empty backbone

    Use

    Empty sgrna plasmid

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6-Sox3gRNA-T1

    Plasmid

    #127777

    Purpose

    Chicken sox3 gRNA target site 1, cloned into the pU6 sgRNA (backbone #92359)

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    sox3gRNAT1
    

    Use

    CRISPR

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMM756

    Plasmid

    #133600

    Purpose

    Part Plasmid for ERG25 sgRNA, Part 234(promoter,coding region, terminator)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    sgERG25
    

    Use

    Synthetic Biology

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge