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pCMV-Xist-PA

Plasmid

#26760

Depositor

Rudolf Jaenisch

Article

Wutz et al Mol Cell. 2000 Apr . 5(4):695-705.

Insert

Xist (Xist Mouse)

Expression

Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pOpen-GBDpol (exo)

Plasmid

#165572

Purpose

Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase

Use

Synthetic Biology

Available Since

Aug. 24, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVhygro-Cre

Plasmid

#34565

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Cre

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

March 28, 2012

Availability

Academic Institutions and Nonprofits only

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pIS2

Plasmid

#12177

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pRIM3-rfp

Plasmid

#74073

Purpose

GentR version of pRIM2 carrying rfp

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

rfp

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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pG14-MS2-GFP

Plasmid

#27117

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2-binding protein

Tags

GFP, HA, and NLS

Expression

Yeast

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pMycVec2

Plasmid

#32361

Depositor

Michael Niederweis

Article

Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

Insert

pJAZ38 ori

Expression

Bacterial

Available Since

Nov. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pAM2195

Plasmid

#40245

Depositor

Susan Golden

Article

Mackey et al Methods Mol Biol. 2007;362:115-29.

Insert

PpsbAI::luxCDE, PpsbAI::luxAB

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pQUAS-luc2

Plasmid

#24337

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

Luciferase

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-mISG15

Plasmid

#12443

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pCAGGS-5HA-mISG15

Plasmid

#12444

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15

Tags

5xHA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pTBS 5Y YIC

Plasmid

#18786

Depositor

Han Htun

Article

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Insert

TRAP binding site

Expression

Mammalian

Available Since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pAd-DsRed

Plasmid

#17515

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

pAd-DsRed

Use

Adenoviral

Expression

Mammalian

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
C17V

Plasmid

#26395

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-17-Venus

Use

Fret standard

Tags

Cerulean and Venus

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pMycVec1

Plasmid

#32360

Depositor

Michael Niederweis

Article

Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

Insert

ColE1 origin replication

Expression

Bacterial

Available Since

Nov. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6His-mISG15

Plasmid

#12445

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15

Tags

6xHis

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pBBR1_DVK_AE

Plasmid

#188946

Purpose

Level 1 destination vector containing pBBR1 origin of replication and CIDAR AE scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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VCV

Plasmid

#27788

Depositor

Steven Vogel

Article

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Venus-Cerulean-Venus

Use

Stoichiometry standard

Expression

Mammalian

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
pSP-G1

Plasmid

#64736

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Partow et al Yeast. 2010 Nov;27(11):955-64. doi: 10.1

Type

Empty backbone

Expression

Yeast

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-181a-1-PGK-GFP

Plasmid

#11376

Depositor

Chang-Zheng Chen

Article

Chen et al Science. 2004 Jan 2. 303(5654):83-6.

Insert

miR-181a-1

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only