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pENTR-HRAS(G12V)-IRES-SNAP-p53DD

Plasmid

#233199

Purpose

Gateway entry vector for HRAS(G12V) and SNAP-p53DD

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-SNAP-p53DD (HRAS, Trp53 Human, Mouse)

Use

Other

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-HRAS(G12V)-IRES-p53DD

Plasmid

#233186

Purpose

Gateway entry vector for HRAS(G12V) and p53DD (RIDD)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-p53DD (HRAS, Trp53 Human, Mouse)

Use

Other

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-p53DD-IRES-HRAS(G12V)

Plasmid

#233187

Purpose

Retroviral expression of p53DD and HRAS(G12V) (DDIR)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

p53DD-IRES-HRAS(G12V) (Trp53, HRAS Human, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-p53DD-IRES-HRAS(G12V)

Plasmid

#233188

Purpose

Gateway entry vector for p53DD and HRAS(G12V) (DDIR)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

p53DD-IRES-HRAS(G12V) (Trp53, HRAS Human, Mouse)

Use

Other

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CNOT_CE_pISceI

Plasmid

#223046

Purpose

Express CNOT2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

CNOT2 (CNOT2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

Plasmid

#227800

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 2 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

Plasmid

#227801

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 3 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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VEGF120TAG

Plasmid

#200946

Purpose

Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF120TAG (Vegfa Mouse)
hPGK-driven Puromycin resistance

Use

Lentiviral

Tags

6x HIS

Mutation

amber stop codon 101 bp from ATG

Promoter

Ef1a and hPGK

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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VEGF164TAG

Plasmid

#200947

Purpose

Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF164TAG (Vegfa Mouse)
hPGK-driven Puromycin resistance

Use

Lentiviral

Tags

6x HIS

Mutation

amber stop codon 101 bp from ATG

Promoter

Ef1a and hPGK

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-HIF1A-Brd 190

Plasmid

#219149

Purpose

Transcription factor HIF1A with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

HIF1A (HIF1A Human)

Use

Lentiviral

Mutation

Codon optimized

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook2_FTS_FHIP2A

Plasmid

#222302

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK2 (HOOK2 Human)
FTS (AKTIP Human)
FHIP2A (FHIP2A Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.BMI1

Plasmid

#224711

Purpose

Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

BMI1 (BMI1 Human)

Tags

6x histidine tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-BMI1-Brd 494

Plasmid

#219388

Purpose

Transcription factor BMI1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

BMI1 (BMI1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-MAZ-Brd 264

Plasmid

#219206

Purpose

Transcription factor MAZ with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

MAZ (MAZ Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-HOPX-Brd 234

Plasmid

#219177

Purpose

Transcription factor HOPX with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

HOPX (HOPX Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-HHEX-Brd 158

Plasmid

#219121

Purpose

Transcription factor HHEX with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

HHEX (HHEX Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-BCL11B-Brd 58

Plasmid

#219038

Purpose

Transcription factor BCL11B with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

BCL11B (BCL11B Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-HCN3 [N141/16R]

Plasmid

#222152

Purpose

Mammalian Expression Plasmid of anti-HCN3 (Mouse). Derived from hybridoma N141/16.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-HCN3 (Mus musculus) recombinant mouse monoclonal antibody. (Hcn3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-KChIP2b K+ channel [K60/73R-2b]

Plasmid

#222169

Purpose

Mammalian Expression Plasmid of anti-KChIP2b K+ channel (Rat). Derived from hybridoma K60/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-KChIP2b K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody. (Kcnip2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdCD80-ST3-His

Plasmid

#210665

Purpose

Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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