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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,509 results for: nls

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Showing: 6241 - 6260 of 6509 results

  1. PB-LAMP1-AlfaTag-APEX2

    Plasmid
    #253247
    Purpose
    Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to AlfaTag for IF or WB detection.
    Depositor
    May Malicdan
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Piggybac transposon
    Tags
    AlfaTag-APEX2
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253247-plasmid-map-sequence-id-505397

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  2. PB-LYST-3xFLAG-2xStrepTag

    Plasmid
    #253253
    Purpose
    Overexpress LYST with a C-terminal 3xFLAG-2xStrepTag.
    Depositor
    May Malicdan
    Insert
    LYST (LYST Human)
    Use
    Piggybac transposon
    Tags
    3xFLAG-2xStrepTag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253253-plasmid-map-sequence-id-505393

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  3. pMM0649

    Plasmid
    #129010
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    129010-plasmid-map-sequence-id-270698

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  4. pMM0655

    Plasmid
    #129016
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    129016-plasmid-map-sequence-id-261247

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  5. pMM0653

    Plasmid
    #129014
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129014-plasmid-map-sequence-id-259453

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  6. pMM0650

    Plasmid
    #129011
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129011-plasmid-map-sequence-id-259446

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  7. pMM0648

    Plasmid
    #129009
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129009-plasmid-map-sequence-id-259442

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  8. pMM0651

    Plasmid
    #129012
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129012-plasmid-map-sequence-id-259438

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  10. PB-LAMP1-3xFLAG-APEX2

    Plasmid
    #253249
    Purpose
    Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to 3xFlag for IF or WB detection.
    Depositor
    May Malicdan
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Piggybac transposon
    Tags
    3xFALG-APEX2
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253249-plasmid-map-sequence-id-503910

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  11. PB-3xFLAG-2xStrepTag-LYST

    Plasmid
    #253254
    Purpose
    Overexpress LYST with an N-terminal 3xFLAG-2xStrepTag.
    Depositor
    May Malicdan
    Insert
    LYST (LYST Human)
    Use
    Piggybac transposon
    Tags
    3xFLAG-2xStrep-Tag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253254-plasmid-map-sequence-id-510252

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  18. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

    Plasmid
    #208974
    Purpose
    Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    B103-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208974-plasmid-map-sequence-id-415835

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Showing: 6241 - 6260 of 6509 results