A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae.
An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes BL, Noyes MB, McClean MN
Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234. PubMed Article
An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes BL, Noyes MB, McClean MN
Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128972 | pMM0281 | Encodes VP16-CIB1 under pADH1 promoter |
| 128973 | pMM0285 | Reporter plasmid, pZF(1BS)-yEVENUS |
| 128974 | pMM0286 | Reporter plasmid, pZF(3oBS)-yEVENUS |
| 128975 | pMM0287 | Reporter plasmid, pZF(3BS)-yEVENUS |
| 128976 | pMM0288 | Reporter plasmid, pZF(3BSc)-yEVENUS |
| 128977 | pMM0289 | Reporter plasmid, pZF(4BSc)-yEVENUS |
| 128978 | pMM0290 | Reporter plasmid, pZF(3BSopp)-yEVENUS |
| 128979 | pMM0317 | Encodes Zif268DBD-CRY2 under pscADH1 |
| 128980 | pMM0320 | Encodes Zif268DBD-CRY2PHR under pscADH1 |
| 128981 | pMM0494 | LEU2 preassembled entry vector |
| 128982 | pMM0519 | Part plasmid for loxP-KIURA3-loxP, Type 6 (yeast marker) part |
| 128983 | pMM0520 | Overexpression plasmid for VP16-CIB1 |
| 128984 | pMM0521 | Expression plasmid for ZIF268DBD-L3-CRY2 |
| 128985 | pMM0528 | Part plasmid for pZF(3BS), Type 2 (promoter) part |
| 128986 | pMM0529 | Part plasmid for Zif268DBD-CRY2, Type 3 (coding sequence) part |
| 128987 | pMM0530 | Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part |
| 128988 | pMM0531 | Part plasmid for VP16-CIB1, Type 3 (coding sequence) part |
| 128989 | pMM0553 | LEU2 integration vector of pZF(3BS)-mRUBY2 |
| 128990 | pMM0554 | LEU2 integrtion vector for VP16-CIB1 |
| 128991 | pMM0557 | LEU2 integration vector for Zif268DBD-CRY2 |
| 128992 | pMM0558 | LEU2 integration vector for Zif268DBD-CRY2PHR |
| 128993 | pMM0617 | loxP-KIURA3-loxP preassembled entry vector |
| 128994 | pMM0619 | Spacer cassette plasmid flanked by ConL2 and ConRE |
| 128995 | pMM0620 | pTEF1-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1 |
| 128996 | pMM0621 | pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1 |
| 128997 | pMM0622 | pTEF1-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2 |
| 128998 | pMM0623 | pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2 |
| 128999 | pMM0624 | pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE |
| 129000 | pMM0625 | pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE |
| 129001 | pMM0626 | pRPL18B-mRUBY2 cassette plasmid flanked by ConL2 and ConRE |
| 129002 | pMM0627 | pTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConRE |
| 129003 | pMM0628 | pRNR2-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1 |
| 129004 | pMM0629 | pRNR2-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2 |
| 129005 | pMM0638 | Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 |
| 129006 | pMM0641 | Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 |
| 129007 | pMM0646 | Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker |
| 129008 | pMM0647 | Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker |
| 129009 | pMM0648 | Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects |
| 129010 | pMM0649 | Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter |
| 129011 | pMM0650 | Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter |
| 129012 | pMM0651 | Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter |
| 129013 | pMM0652 | Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects |
| 129014 | pMM0653 | Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter |
| 129015 | pMM0654 | Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter |
| 129016 | pMM0655 | Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter |
| 129017 | pMM0656 | pTEF1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2 |
| 129018 | pMM0657 | pRPL18B-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2 |
| 129019 | pMM0658 | pREV1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2 |