We narrowed to 15,115 results for: Pol

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pCrysβ:ECFP-LexOP:mCherry-KrasG12V

Plasmid

#89888

Purpose

Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-KRasG12V

Use

Unspecified

Tags

mCherry

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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GfaABC1D BARK D110A p2A mCherry

Plasmid

#117692

Purpose

GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

AAV

Tags

HA / FLAG

Mutation

D110A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin S3A

Plasmid

#50857

Purpose

expresses constituvely active cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
pmRFP-N1 human cofilin S3E

Plasmid

#50858

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pEMS1994

Plasmid

#49124

Purpose

AAV plasmid with S100B (Ple266) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple266-iCre

Use

AAV

Expression

Mammalian

Promoter

S100B

Available Since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSCALPs-HA-NFAT1 (4-460)-GFP

Plasmid

#88879

Purpose

Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)

Use

Lentiviral

Tags

GFP fusion protein and HA tag

Expression

Mammalian

Mutation

amino acids 4-460 only; L78P & L287P *see bel…

Promoter

SFFV (spleen focus forming virus)

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS2158

Plasmid

#70119

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-EmGFP

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMT-Mef2c-tPT2A-GFP

Plasmid

#111771

Purpose

Bicistronic construct: Co-express two genes by 2A peptides

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Insert

Mef2c-tPT2A-GFP (Mef2c Mouse)

Use

Cloning intermediate

Tags

GFP

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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CAG FLEX BARK D110A p2A mCherry

Plasmid

#117694

Purpose

CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

Cre/Lox

Tags

HA / FLAG

Mutation

D110A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1 TMEM192-Flag

Plasmid

#112742

Purpose

express pLJM1 TMEM192-Flag

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

TMEM192-Flag (TMEM192 Human)

Use

Lentiviral

Tags

Flag

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEF myc Gal cyclin T1 (1-726) (P#1026)

Plasmid

#14626

Depositor

Matija Peterlin

Article

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

cyclin T1 (CCNT1 Human)

Tags

Gal and myc

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pQHA-USP7 WT puroR

Plasmid

#46753

Purpose

Retroviral vector that expresses wild type HA-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

Plasmid

#110201

Purpose

Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

LexPR transactivator - 2A - Cerulean

Tags

Cerulean

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2159

Plasmid

#70120

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 12, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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mEos3-Rac1

Plasmid

#92039

Purpose

Expresses mEos3-Rac1 fusion protein

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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mEos3-dnRac1

Plasmid

#92040

Purpose

Expresses mEos3 fusion with dnRac1 (T17N).

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

T17N (dominant negative)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pQHA-USP7 CS puroR

Plasmid

#46754

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1995

Plasmid

#49125

Purpose

AAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple94-iCre

Use

AAV

Expression

Mammalian

Promoter

GPR88

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5FRT/TO-Strep/HA-UBE2J1

Plasmid

#124669

Purpose

Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2J1 (UBE2J1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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