We narrowed to 28,294 results for: pET

1. pET23b-Casp7 C186A-His

   Plasmid

   #11826

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 7 catalytic mutant (CASP7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C186A

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-Hook3 aa 1-160-GCN4

   Plasmid

   #74608

   Purpose

   Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-160 (HOOK3 Human)
   

   Tags

   6x His, GCN4, Strep II, and superfolder GFP

   Expression

   Bacterial

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3535 pET15b Bid p22

   Plasmid

   #8784

   Depositor

   Stanley Korsmeyer

   Article

   Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

   Insert

   Bid p22 (Bid Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pET-30a(+)-Syk-tSH2-PM

   Plasmid

   #111271

   Purpose

   express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
   

   Expression

   Bacterial

   Mutation

   Y130E mutation to mimic phosphorylation

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-his6-HA3-CLIP

   Plasmid

   #188942

   Purpose

   T7 expression vector for making his6- and HA3-tagged CLIP protein

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   his6-HA3-CLIP
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-At4CL-CmCHS-CmCHI

   Plasmid

   #73395

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   At4CL
   CmCHS
   CmCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30a-SARS-CoV2-ORF8

   Plasmid

   #165089

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET42b-A3A-PBE-ΔUGI

   Plasmid

   #119771

   Purpose

   To express the recombinant APOBEC3A in vitro

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS
   

   Tags

   6*HIS and NLS

   Expression

   Bacterial

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-22HT-MSI2(8-193)

   Plasmid

   #60356

   Purpose

   Express the RBD of human MSI2 in bacterial cells for protein purification

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   human MSI2 (8-193) (MSI2 Human)
   

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   contains amino acids 8-193 of MSI2

   Promoter

   T7

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet RagA-RagC(S75N)

    Plasmid

    #99645

    Purpose

    Bacterial expression of RagA-RagC(S75N)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    S75N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6-Pc4CL-CmCHS-MsCHI

    Plasmid

    #73398

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    CmCHS
    MsCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. WT N-His Ana GvpC in pET28a

    Plasmid

    #153294

    Purpose

    Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (N-terminal his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added a hexahistidine tag after the methionine at…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet16b ALIX Bro1 (1-359)

    Plasmid

    #80641

    Purpose

    Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    ALIX Bro1, residues 1-359 (PDCD6IP Human)
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Residues 1-359

    Promoter

    T7

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pet151D-TOPO BROX 1-411;

    Plasmid

    #89602

    Purpose

    Expresses full length human Brox in bacteria; internal ID WISP11-297

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pF714-pET28a-Hs-FL-METTL20-NHis wt

    Plasmid

    #85115

    Purpose

    expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pET51b-Strep-hAGT-His

    Plasmid

    #167276

    Purpose

    Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    hAGT (AGT Human)
    

    Tags

    His x10 and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-sfGFP[D134TAG+N150TAA]

    Plasmid

    #164582

    Purpose

    superfolder GFP with D134TAG and N150TAA mutations

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-134TAG+150TAA
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAA Mutations

    Promoter

    T7

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET 15b-UbcH5c/2418

    Plasmid

    #11473

    Depositor

    Allan Weissman

    Article

    Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

    Insert

    UbcH5c (UBE2D3 Human)
    

    Expression

    Bacterial

    Mutation

    n/a

    Available Since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pET28a(+) 6xHis-TEV-IFIT5

    Plasmid

    #53560

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET-30a(+)-Syk-tSH2

    Plasmid

    #111269

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge