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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 28,830 results for: cas9 genes

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Showing: 6281 - 6300 of 28830 results

  14. lenti-EF1a-dCas9-KRAB-MeCP2-H2B-mCherry

    Plasmid
    #242171
    Purpose
    Lentiviral expression of dCas9-KRAB-MeCP2 with H2B-mCherry reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB-MeCP2; T2A-H2B-mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Feb. 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    242171-plasmid-map-sequence-id-499156

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  16. pSA144 HBB_IVS2-Cas9-BFP with core cHS4 insulators

    Plasmid
    #249154
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    HBB_IVS2-Cas9-TagBFP with core cHS4 insulators (HBB S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249154-plasmid-map-sequence-id-496518

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  18. pEvolvR-mCherry-eNme2.C.NR-nCas9(D16A)-PolI5MΔ

    Plasmid
    #249077
    Purpose
    Expresses nucleus-localized eNme2.C.NR-nCas9 (D16A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    eNme2.C.NR-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249077-plasmid-map-sequence-id-496316

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  20. pCMV-SpCas9-VRQR(+L1111R)-P2A-EGFP (LRF167)

    Plasmid
    #242673
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L1111R mutations in SpCas9(L1111R/D1135V/G…
    Promoter
    CMV and T7
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242673-plasmid-map-sequence-id-494379

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Showing: 6281 - 6300 of 28830 results