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pAAV9-hSyn-iDianiSnFR_ER

Plasmid

#177758

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iDianiSnFR_ER

Use

AAV

Promoter

hSyn

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCyt_F_SnFR_PM

Plasmid

#177755

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCyt_F_SnFR_PM

Use

AAV

Promoter

hSyn

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCytSnFR_PM

Plasmid

#177753

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCytSnFR_PM

Use

AAV

Promoter

hSyn

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCytSnFR_ER

Plasmid

#177752

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCytSnFR_ER

Use

AAV

Promoter

hSyn

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn iCyt_BrEt_SnFR_ER

Plasmid

#177756

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_ER

Use

AAV

Promoter

hSyn

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCyt_F_SnFR_ER

Plasmid

#177754

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCyt_F_SnFR_ER

Use

AAV

Promoter

hSyn

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_P229L-talin1 (1-2541)

Plasmid

#166113

Purpose

expresses talin-1-P229L in mammalian cells

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

P229L-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_RXRG_Hormone-recep

Plasmid

#110019

Purpose

Protein expression and purification of RXRG_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RXRG_Hormone-recep (RXRG Human)

Expression

Bacterial

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr2

Plasmid

#136760

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr2

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr2 (Or6a2 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr653

Plasmid

#136751

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr653

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr653 (Olfr653 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr160

Plasmid

#136703

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr160

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr160 (Olfr160 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr19

Plasmid

#136705

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr19

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr19 (Olfr19 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo(Orange)

Plasmid

#140157

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Orange)

Plasmid

#140158

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Crimson)

Plasmid

#140156

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS attB V5 dDispatched WT HA

Plasmid

#126411

Purpose

Expression of wild type dDispatched HA in Drosophila.

Depositor

Stacey Ogden

Publication

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI129

Plasmid

#126210

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1962

Plasmid

#82572

Purpose

High copy number plasmid containing Ple303 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple303

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1963

Plasmid

#82573

Purpose

High copy number plasmid containing Ple304 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple304

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMS1961

Plasmid

#82571

Purpose

High copy number plasmid containing Ple302 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple302

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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