Characterizing a strong pan-muscular promoter (Pmlc-1) as a fluorescent co-injection marker to select for single-copy insertions.
El Mouridi S, Peng Y, Frokjaer-Jensen C
MicroPubl Biol. 2020 Sep 3;2020. doi: 10.17912/micropub.biology.000302. PubMed Article
El Mouridi S, Peng Y, Frokjaer-Jensen C
MicroPubl Biol. 2020 Sep 3;2020. doi: 10.17912/micropub.biology.000302. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159794 | pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR] | Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans |
| 159795 | pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR] | Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans |
| 159888 | pCFJ2113 - [4-1] - Promoter - Pmlc-1 (w ATG) | Three-fragment Gateway compatible promoter for expression in all muscles of C. elegans |
| 159889 | pSEM223 - [4-1] - Promoter - Pmlc-2(no_start) | Three-fragment Gateway compatible promoter for expression in all muscles of C. elegans |
| 159895 | pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR] | Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans |
| 159896 | pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR] | Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans |
| 159897 | pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR] | Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans |
| 159898 | pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR] | Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans |
| 159899 | pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR] | Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans |
| 159900 | pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR] | Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans |