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pDA095

Plasmid

#131391

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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p35S:Citrine-TurboID-3xHA

Plasmid

#199243

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID

Tags

3xHA and TurboID-3xHA

Expression

Plant

Promoter

CaMV 35S

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTW2066

Plasmid

#236575

Purpose

ISYmu1 PDS3 gRNA2 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMK025

Plasmid

#236572

Purpose

Cloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into ISYmu1 plant expression vector

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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GB-NES

Plasmid

#61017

Purpose

ddGFP B-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP B

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTW2082

Plasmid

#236576

Purpose

ISYmu1 PDS3 gRNA12 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ223 (dAsCpf1-SRDX)

Plasmid

#86209

Purpose

Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

dAsCpf1

Use

Gateway compatible cpf1 vectors

Tags

NLS and SRDX repressor and NLS

Expression

Plant

Mutation

D908A

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDA056

Plasmid

#131387

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Expression

Mammalian

Promoter

TRE3G

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDA077

Plasmid

#131388

Purpose

L1RP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pUltra-sY

Plasmid

#82417

Purpose

Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

Depositor

Chang Liu

Article

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Methanococcus jannaschii aaRS for sulfotyrosine

Expression

Bacterial

Mutation

Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

Promoter

tacI

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPD0029_pIVTRup(BsmBI)_SpCas9

Plasmid

#242535

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

SpCas9

Use

CRISPR

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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mCardinal-pBAD

Plasmid

#54800

Purpose

Localization: Protein Expression Vector , Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

6xHis and mCardinal

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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CAG-BFP-sesRNA-tTA2-WPRE-pA

Plasmid

#192070

Purpose

Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3B-BE3

Plasmid

#113411

Purpose

Expresses hA3B-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3B-BE3 (APOBEC3B Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP2A-IRES-GFP

Plasmid

#220352

Purpose

Expresses EBV latent gene LMP2A in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BGLF5

Plasmid

#195813

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF5

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF5 (BGLF5 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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Integrin β8-GFP

Plasmid

#205093

Purpose

Express GFP-tagged human integrin β8 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 8 (ITGB8 Human)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOBD2

Plasmid

#196826

Purpose

Yeast two-hybrid ; Gal4 DNA binding domain

Depositor

Stan Fields

Article

Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

Type

Empty backbone

Tags

Gal4 DNA binding domain

Expression

Yeast

Promoter

ADH

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRibo-Sec-APEX2m

Plasmid

#176844

Purpose

Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-Sec-APEX2m

Use

Inducible

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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