We narrowed to 13,416 results for: sequence
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Plasmid#91689PurposeMMTV-LTR promoter/enhancer with rabbit beta-globin intron and bovine growth hormone poly-A sequence to enhance transgene expressionDepositorInsertMMTV LTR cloned into KbpA vector
ExpressionMammalianPromoterMMTV LTRAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS_36x-601_MA1+MA2+MA3
Plasmid#114363Purpose36x Widom 601 nucleosome sequenceDepositorInsert36X601-MA1_2_3
UseUnspecifiedExpressionMammalianAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p8.9 NdeltaSB D185K/D186L
Plasmid#132931Purposep8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequenceDepositorInsertgag and pol D185K/D186L
UseLentiviralMutationD185K/D186LAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
AiRTi
Plasmid#214068PurposeFluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRTX_ctrl
Plasmid#214069PurposeFluorescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationRemnant of linker sequence present at the end of …Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRT
Plasmid#214067PurposeFluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1-iRFP-iLid-CaaX
Plasmid#155229Purposemembrane markerDepositorInsertiRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control
TagsiRFP-iLidExpressionMammalianAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsTAB182_K
Plasmid#146777PurposeMammalian Expression of HsTAB182DepositorInsertHsTAB182 (TNKS1BP1 Human)
ExpressionMammalianMutation2 silent mutations compared to the sequence given…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-72:TFAM-mEGFP
Plasmid#159745PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human TFAMDepositorInsertTFAM Homology Arms with linker-mEGFP (TFAM Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceOct. 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorInsertLasI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pREP
Plasmid#51491PurposepMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virusDepositorInsertRep/RepA
UsePlant expression t-dnaMutationnonePromoter2x35SAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxI
Plasmid#193626PurposeConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.DepositorInsertLuxI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKT-iRFP-KAN
Plasmid#64687PurposepKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.DepositorInsertiRFP
ExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB1610_pRR-Puro
Plasmid#65853PurposePuromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9DepositorInsertsplit puromycin N-acetyltransferase coding sequence
UseCRISPR and TALENExpressionMammalianPromoterSV40Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
BTV
Plasmid#84771Purposebidirectional tetracycline-regulated viral reporter that measures the effects of 3' UTR sequences on mRNA and protein productionDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AiRT_RTctrl
Plasmid#214070PurposeFluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-mRFP1-Human UTROPHIN_1-261
Plasmid#225052PurposeExpress in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and RFP1 (UTRN Homo Sapiens, Human)
UseLentiviralTagsHuman UTROPHIN 1-261 and mRFP1ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26739-R…PromoterCMV promoter and enhancerAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EGFP-Human-UTROPHIN_1-261
Plasmid#222393PurposeExpress in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and EGFP (UTRN Homo Sapiens, Human)
UseLentiviralTagsEGFP and Human UTROPHIN 1-261ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26737-G…PromoterCMV promoter and enhancerAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-10XGP41-J9
Plasmid#202005PurposeMoClo level 0 dCas9-10XGP41 coding sequence moduleDepositorInsertdCas9-10XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only