We narrowed to 9,310 results for: yeast expression

1. pML104-HygMx4-URA3-2

   Plasmid

   #232885

   Purpose

   Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   URA3 gRNA (URA3 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG426-GAL-Prepro-3x-Adan

   Plasmid

   #181733

   Purpose

   Galactose inducible expression of Prepro-3x-Adan

   Depositor

   Alejandro Chavez

   Insert

   Prepro-3x-Adan
   

   Expression

   Yeast

   Available Since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG416-GAL-RNQ1

   Plasmid

   #181706

   Purpose

   Galactose inducible expression of RNQ1

   Depositor

   Alejandro Chavez

   Insert

   RNQ1 (RNQ1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUSE-URA3-Sec7-msYFP2

   Plasmid

   #218969

   Purpose

   Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2

   Depositor

   Benjamin Glick

   Insert

   SEC7-msYFP2 (SEC7 Budding Yeast)
   

   Tags

   msYFP2

   Expression

   Yeast

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGR475

   Plasmid

   #213784

   Purpose

   Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker

   Depositor

   Chang Liu

   Insert

   WT TP-DNAP1 (recoded)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLK89 - Edit-directing plasmid cloning insert

   Plasmid

   #98846

   Purpose

   Contains the insert used for step 2 of edit-directed plasmid cloning

   Depositor

   Leonid Kruglyak

   Insert

   KanR
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Changed sequence to remove a MluI cut site

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pREP4xV5_ccdB

   Plasmid

   #79016

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREP3xV5_ccdB

   Plasmid

   #79015

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS313_MSN5-LY00018

   Plasmid

   #232903

   Purpose

   Plasmid carrying MSN5 from LY00018 (YJM975) with its native promoter and terminator.

   Depositor

   Jolanda Van Leeuwen

   Insert

   MSN5 (MSN5 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   G1563A

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. HCP2

    Plasmid

    #166104

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

    Depositor

    Matthias Heinemann

    Insert

    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. HCP3

    Plasmid

    #166105

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

    Depositor

    Matthias Heinemann

    Insert

    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HCP7

    Plasmid

    #166109

    Purpose

    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.

    Depositor

    Matthias Heinemann

    Insert

    Whi5-sg106 (WHI5 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. brr2-C520R pRS313

    Plasmid

    #111412

    Purpose

    brr2-C520R (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    C520R aa mutation

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. brr2-N1104A pRS313

    Plasmid

    #111414

    Purpose

    brr2-N1104A (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    N1104A aa mutation

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. brr2-R1107L pRS313

    Plasmid

    #111416

    Purpose

    brr2-R1107L (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    R1107L aa mutation

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pREP81xV5_ccdB

    Plasmid

    #79019

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction

    Depositor

    Pernilla Bjerling, Göran Akusjärvi

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pREP41xV5_ccdB

    Plasmid

    #79017

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection

    Depositor

    Pernilla Bjerling, Göran Akusjärvi

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pREP82xV5_ccdB

    Plasmid

    #79020

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

    Depositor

    Pernilla Bjerling, Göran Akusjärvi

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pREP42xV5_ccdB

    Plasmid

    #79018

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

    Depositor

    Pernilla Bjerling, Göran Akusjärvi

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK275

    Plasmid

    #73363

    Purpose

    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)

    Depositor

    T. J. Kappock

    Insert

    delta-8 sphingolipid desaturase
    

    Expression

    Yeast

    Mutation

    missing residues 1-6

    Promoter

    GAL1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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