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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 32,362 results for: Nov

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Showing: 6341 - 6360 of 32362 results

  1. eSpCas9(1.1)

    Plasmid
    #138555
    Purpose
    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    eSpCas9(1.1)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138555-plasmid-map-sequence-id-274761

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  9. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid
    #183502
    Purpose
    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.
    Depositor
    Wayne Tilley
    Publication
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    mKate2
    BSD
    Use
    Lentiviral and Luciferase
    Tags
    P2A linker and SV40 NLS with GGS linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183502-plasmid-map-sequence-id-362519

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  11. pBBR_Cas9_sp1

    Plasmid
    #154851
    Purpose
    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Spcas9 gene
    sgRNA scaffold
    Use
    CRISPR
    Tags
    6xHis
    Mutation
    Codon harmonized variant for expression in Rhodob…
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154851-plasmid-map-sequence-id-309592

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  12. pMTMVi-N

    Plasmid
    #118756
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.
    Depositor
    Jose-Antonio Daros
    Publication
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118756-plasmid-map-sequence-id-236109

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  13. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70703-plasmid-map-sequence-id-127765

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Showing: 6341 - 6360 of 32362 results