We narrowed to 11,576 results for: Vars;

1. pAG8Ha-His-EWSR1-359-549

   Plasmid

   #195868

   Purpose

   Expresses the RRM-RG2-ZnF (359-549) of EWS

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWS 359-549 (EWSR1 Human)
   

   Use

   Tags

   His8-linker-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. macroH2A1.1-F192V-GFP (pc5114)

   Plasmid

   #223437

   Purpose

   Mutation phenylalanine 192 to valine (F192V) in macroH2A1.1-GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A1.1-F192V (Macroh2a1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti HsATP13A2 P652_E653 del

   Plasmid

   #213700

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

   Depositor

   Veerle Baekelandt

   Article

   Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

   Insert

   human ATP13A2 P652_E653 del (ATP13A2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT, D962N

   Promoter

   CMV

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINVHH_A8_His

   Plasmid

   #201718

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH_A8 (S lama glama (llama))
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINVHH_A10-5_His

   Plasmid

   #201720

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH_A10-5 (S lama glama (llama))
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINVHH_B5-5_His

   Plasmid

   #201721

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH B5-5 (S lama glama (llama))
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-enCas12a-A3A-N57G

   Plasmid

   #193651

   Purpose

   Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLb-enCas12a-hA3A-N57G, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

   Plasmid

   #201576

   Purpose

   Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutations S172A, M173A and V195A

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Stx5L(m55V)-GFP

   Plasmid

   #209845

   Purpose

   Expresses Stx5 M55V mutant fused to GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   STX5 (STX5 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Methionine 55 to Valine, causes loss of S…

   Promoter

   CMV

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. B-HypaR-SpCas9

    Plasmid

    #126764

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Hypa2SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A; N692A; M694A; Q695A; H698A; amino acids 10…

    Promoter

    CBh

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ERAP1-rs27895(C)

    Plasmid

    #206142

    Purpose

    Expresses the rs27895-C allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. K365-iLid

    Plasmid

    #188456

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Zvonimir Dogic

    Article

    Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

    Insert

    Kinesin 1-365 (Khc Fly)
    

    Use

    Tags

    6xHis and iLid

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-365

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. K365-micro

    Plasmid

    #188457

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Zvonimir Dogic

    Article

    Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

    Insert

    Kinesin 1-365 (Khc Fly)
    

    Use

    Tags

    6xHis-MBP-TEV and SspB micro

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-365

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBT-RP8.3

    Plasmid

    #189848

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    mRFP and tagBFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-EGFP-C1-HseIF4G1-L640AV646A_AD

    Plasmid

    #148520

    Purpose

    Mammalian Expression of HseIF4G1-L640AV646A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-L640AV646A (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD

    Plasmid

    #148521

    Purpose

    Mammalian Expression of HseIF4G1-LIVVtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-LIVVtoAAAA (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD

    Plasmid

    #148522

    Purpose

    Mammalian Expression of HseIF4G1-Y619AL624AL625A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-Y619AL624AL625A (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RAT +D122H

    Plasmid

    #187426

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed aspartic acid at 129 for histidine

    Promoter

    PH and p10

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. RAT +R111E

    Plasmid

    #187425

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed arginine at 118 to glutamic acid

    Promoter

    PH and p10

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IL2-mCherry-rGBD in pmCherryN1

    Plasmid

    #187286

    Purpose

    Express IL2-Rhotekin GTPase-binding domain-mCherry

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    interleukin-2 receptor alpha-subunit, Rhotekin (IL2, Rtkn Mouse, Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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