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NSK250 CMV-TO-SCN1A (exons 20, 20N, 21)

Plasmid

#247285

Purpose

Expresses human SCN1a exons 20, 20N, 21

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

SCN1a exons 20, 20N, 21 (SCN1A Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK249 CMV-TO-SCN1A (exons 20, 21)

Plasmid

#247284

Purpose

Expresses human SCN1a exons 20, 21

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

SCN1a exons 20, 21 (SCN1A Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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G-GECO1.2 (F90L)

Plasmid

#240471

Purpose

Low-affinity green GECI (cytosolic)

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

G-GECO1.2 (F90L)

Expression

Mammalian

Mutation

Mutated phenylalanine 359 to leucine (GECO number…

Promoter

CMV

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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SM268_EF1A-dHyperLbCas12a-P300-T2A-EGFP

Plasmid

#244206

Purpose

Mammalian expression of dHyperLbCas12a-P300 with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM269_EF1A-dHyperLbCas12a-P300-T2A-BlastR

Plasmid

#244207

Purpose

Mammalian expression of dHyperLbCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM281_EF1A-dEnAsCas12a-P300-T2A-EGFP

Plasmid

#244216

Purpose

Mammalian expression of dEnAsCas12a-P300 with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-miRFP703-dSal-VP16minADx3-hCRY2-NLS

Plasmid

#241843

Purpose

Expresses an actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

VP16

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

VP16 minimal activation domain x 3

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.1

Plasmid

#235053

Purpose

Expresses Tad-CBE2.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE2.5

Plasmid

#235054

Purpose

Expresses Tad-CABE2.5 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE2.5-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.1

Plasmid

#235056

Purpose

Expresses Tad-CBE3.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.2

Plasmid

#235057

Purpose

Expresses Tad-CBE3.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE1.1

Plasmid

#235051

Purpose

Expresses Tad-CABE1.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE1.2

Plasmid

#235052

Purpose

Expresses Tad-CABE1.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CT751

Plasmid

#233657

Purpose

Dox-inducible VP64-MyoD

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

VP64-MyoD

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT754

Plasmid

#233659

Purpose

FLEx switch with tTA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

tTA FLEx switch

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMO819

Plasmid

#232072

Purpose

Expresses Cytosine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Cytosine Base Editor

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1-alpha core promoter

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMO1170

Plasmid

#232073

Purpose

Expresses Adenine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Adenine Base Editor

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1-alpha core promoter

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSCD-GSDMA-C

Plasmid

#226235

Purpose

Express GSDMA-C domain

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

Gasdermin A (Gsdma3 Mouse)

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSCD-cTEVP-RBD

Plasmid

#226233

Purpose

Express cTEVP-RBD fusion

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

cTEVP-RBD (TEVgp1 Tobacco etch virus)

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSCD-nTEVP-RBD

Plasmid

#226232

Purpose

Express nTEVP-RBD fusion

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

nTEVP-RBD (TEVgp1 Tobacco etch virus)

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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