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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,416 results for: sequence

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Showing: 6361 - 6380 of 13416 results

  4. pDB4283

    Plasmid
    #98702
    Purpose
    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    gRNA PCR template
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98702-plasmid-map-sequence-id-182828

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  5. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Mutation
    Contains D10A and H840A mutations
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122836-plasmid-map-sequence-id-239365

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  9. lentiMutB3GNT5-blast

    Plasmid
    #208380
    Purpose
    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nt 922 c->g (PAM site inactivation)
    Promoter
    EF-1 alpha core promoter
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208380-plasmid-map-sequence-id-414094

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  12. pQE30-TEV-pro-conA

    Plasmid
    #159527
    Purpose
    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.
    Depositor
    Joshua Mylne
    Article
    Nonis et al bioRxiv. 2020.
    Insert
    Canavalia ensiformis pro-concanavalin A
    Tags
    Six-Histidine tag and tobacco etch virus (TEV) pr…
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    159527-plasmid-map-sequence-id-317076

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  14. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212120-plasmid-map-sequence-id-444184

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Showing: 6361 - 6380 of 13416 results