We narrowed to 22,955 results for: CRISPR

1. pCRISPR-cBEST-v2-kasOP*

   Plasmid

   #209447

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from kasOP* promoter

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR/eCas9-GPIDAF

   Plasmid

   #213710

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR/eCas9-GPIBY55

   Plasmid

   #213709

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPRv2-bleo-COX1-4

   Plasmid

   #184722

   Purpose

   COX1-KO in MDCK

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the dog COX1 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2-hyg2-COX2-4

   Plasmid

   #184723

   Purpose

   COX2-KO in MDCK

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the dog COX2 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2-bleo-PTGER2

   Plasmid

   #184724

   Purpose

   EP2-KO in MDCK

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the dog EP2 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2-bleo-PLA2G4A_3

   Plasmid

   #184725

   Purpose

   cPLA2-KO in MDCK

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the dog cPLA2 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPRv2-hyg2-PTGER4

   Plasmid

   #184726

   Purpose

   EP4-KO in MDCK

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the dog EP4 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPR-v2 hygro sgUCK1

   Plasmid

   #211524

   Purpose

   Deletes UCK1

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   sgUCK1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAf-CRISPR-ctfR1

    Plasmid

    #207992

    Purpose

    CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

    Insert

    ctfR1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus flavus U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPRv2-bleo-ErbB4

    Plasmid

    #197353

    Purpose

    A knock-out vector for dog ErbB4.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPRa_all-in-one_Bip gRNA1

    Plasmid

    #183696

    Purpose

    Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    gRNA targeting chinese hamster Bip (Hspa5)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR2_+10D+10U

    Plasmid

    #149394

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted upstream and downstream of the IHFp…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPRv2-bleo-dEgf

    Plasmid

    #173840

    Purpose

    A knock-out vector for the dog Egf

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2-puro-dHbegf

    Plasmid

    #173841

    Purpose

    A knockout vector for the dog Hbegf.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiCRISPRv2-neo-dTgfa

    Plasmid

    #173842

    Purpose

    A knockout vector for the dog Tgfa.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPRv2-hyg2-dEreg

    Plasmid

    #173843

    Purpose

    A knockout vector for the dog Ereg.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPRv2-puro-dEgfr

    Plasmid

    #173844

    Purpose

    A knockout vector for the dog Egfr.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p8116 LentiCRISPR v2 sgPTPN14-4

    Plasmid

    #163316

    Purpose

    Expresses Cas9 and a human PTPN14-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    sgPTPN14-4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p8115 LentiCRISPR v2 sgPTPN14-3

    Plasmid

    #163314

    Purpose

    Expresses Cas9 and a human PTPN14-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgPTPN14-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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