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We narrowed to 2,070 results for: gem

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Showing: 621 - 640 of 2070 results

  4. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74492-plasmid-map-sequence-id-140493

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  8. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Publication
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85165-plasmid-map-sequence-id-354765

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  10. pRN120

    Plasmid
    #160696
    Purpose
    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator
    Depositor
    Daniel Voytas
    Publication
    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CmYLCV::Luc+::AtHSP
    Nos::WUS2::PinII
    AtUbi10::STM::35S
    Use
    Luciferase; Gemini viral replicon, plant developm…
    Promoter
    AtUbi10, CmYLCV, and Nos
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160696-plasmid-map-sequence-id-316629

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  11. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Expression
    Bacterial and Mammalian
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113631-plasmid-map-sequence-id-222216

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  13. Dat - EGFP - Tol2

    Plasmid
    #85418
    Purpose
    for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene
    Depositor
    Marc Ekker
    Publication
    Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:
    Insert
    dopamine transporter (slc6a3 Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Promoter
    dat
    Available since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. Stx1a-12aa-mCer

    Plasmid
    #53236
    Purpose
    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Syntaxin 1A (Stx1a Rat)
    Tags
    12aa-linker and mCerulean
    Expression
    Mammalian
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pSFVCs-lacZ

    Plasmid
    #92076
    Purpose
    This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.
    Depositor
    Henrik Garoff
    Publication
    Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C-lacZ
    Use
    C-lacz expression plasmid, for run-off transcript…
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92076-plasmid-map-sequence-id-181625

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  17. pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]

    Plasmid
    #247262
    Purpose
    Express ISCro4 recombinase and bridge RNA
    Depositor
    Patrick Hsu
    Publication
    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1
    Insert
    pNP2922 - ISCro4 bRNA1-179 C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101-111) (TBL4+DBL3)
    Expression
    Mammalian
    Mutation
    C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101…
    Available since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    247262-plasmid-map-sequence-id-490480

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  20. 1.3xWT HBV-Luciferase

    Plasmid
    #61549
    Purpose
    The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core
    Depositor
    Yosef Shaul
    Publication
    Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    1.3 times of the HBV genome with luciferase within the Core ORF
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Substitution of HBV sequences with luciferase ORF…
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61549-plasmid-map-sequence-id-212455

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Showing: 621 - 640 of 2070 results