We narrowed to 6,849 results for: GFP expression plasmids

1. pCIG1_UL136_19kDa_EE

   Plasmid

   #74951

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Glu-Glu tag (EYMPME)

   Expression

   Mammalian

   Mutation

   delta 1-127

   Promoter

   CMV, lentiviral LTR

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCIG1_UL136_23kDa_Myc

   Plasmid

   #74949

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   HA Tag (YPYDVPDYA)

   Expression

   Mammalian

   Mutation

   delta 1-99 M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIG1_UL136_25kDa_Myc

   Plasmid

   #74954

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)

   Expression

   Mammalian

   Mutation

   delta 1-77, M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRUPATH Triple Gaq

   Plasmid

   #196058

   Purpose

   Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaQ-RLuc8 (GNAQ Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TRUPATH Triple GaoA

   Plasmid

   #196051

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaoA-RLuc8
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRUPATH Triple Ga13

   Plasmid

   #196062

   Purpose

   Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha13-RLuc8 (GNA13 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRUPATH Triple GaoB

   Plasmid

   #196052

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaoB-RLuc8
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRRL_U2AF1_S34F_mCherry

   Plasmid

   #84019

   Purpose

   expression of mutant U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   S34F

   Promoter

   PGK

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRRL_U2AF1_S34Y_mCherry

   Plasmid

   #84018

   Purpose

   expression of mutant U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   S34Y

   Promoter

   PGK

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

10. FH95pUP95S73AGW (F2)

    Plasmid

    #74039

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusion

    Depositor

    Robert Malenka, Bernardo Sabatini, Weifeng Xu

    Article

    Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    S73A

    Promoter

    H1

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

11. FH95pUP95S73DGW (F3)

    Plasmid

    #74040

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusion

    Depositor

    Robert Malenka, Bernardo Sabatini, Weifeng Xu

    Article

    Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    S73D

    Promoter

    H1

    Available since

    Availability

    Academic Institutions and Nonprofits only

12. 5aa SunTag VP64 EVD

    Plasmid

    #115483

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEG302 22aa SunTag NtDRMcd nog

    Plasmid

    #115488

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEG302 22aa SunTag VP64 nog

    Plasmid

    #120251

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEG302 22aa SunTag VP64 gRNA4 (FWA)

    Plasmid

    #120249

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)

    Plasmid

    #115486

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEG302 22aa SunTag VP64 g4+g17

    Plasmid

    #120252

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEG302 5aa SunTag VP64 nog

    Plasmid

    #115480

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEG302 22aa SunTag NtDRMcd SUP

    Plasmid

    #115489

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEG302 5aa SunTag VP64 gRNA4 (FWA)

    Plasmid

    #115481

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge