We narrowed to 7,362 results for: Ank
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Plasmid#205003PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLM494
Plasmid#100539PurposeCEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.DepositorInsertsGGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase
UseCre/Lox and Synthetic BiologyExpressionYeastPromoterPGK1, TDH3, TIP1, and ZEO1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-mScarlet
Plasmid#227274PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc' CST-1
Plasmid#66859PurposeExpress Myc-tagged C.elegans CST-1 in mammalian cellsDepositorInsertCST-1 (cst-1 Nematode)
TagsMycExpressionMammalianMutationS48N, H50R, S257P and V340A mutations in CST-1 co…PromoterCMVAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAT-PB-CD2APtk
Plasmid#86004Purposehomology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassetteDepositorInsertDsRedEx_T2A_puro_dTK
UsePiggybac exciseableExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 catalytic
Plasmid#227201PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationaa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-2
Plasmid#210388PurposeAll-in-one cumate-based inducible CRISPRi plasmid for use in StreptomycesDepositorInsertsdCas9
CymR
sgRNA cassette
UseCRISPR; Integrative vector for use in streptomycesMutationBsaI-flanked RFP cassette inserted in place of sp…PromoterSP11 and SP30Available SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBEC6
Plasmid#187598PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC5Kan-P2A
Plasmid#51814PurposeP2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single messageDepositorInsertP2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
UseShuttle vector for insect expression vectorsMutationcodons altered for Drosophila and to eliminate re…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH7WG2B-OsMIR390-B/c
Plasmid#61465PurposePlant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
ExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…PromoterOsUBIAvailable SinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmROSA26-attP50-Neo-mKate2-3xNLS-attP50
Plasmid#183609PurposeTargeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.DepositorInsertsNeo
mKate2-3xNLS
UseMouse TargetingTags3xNLSAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
ExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3HA-hMIB2
Plasmid#33312DepositorAvailable SinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPEE47
Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
ExpressionYeastPromoterTEF1Available SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE46
Plasmid#139425PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.DepositorInsertmRuby3
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtMIR390a-B/c
Plasmid#51776PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursorDepositorInsertAtMIR390a-B/c
UsePlant expressionMutationA. thaliana MIR390a precursor sequence including …Available SinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-pX330-Cas9-Blast
Plasmid#159741PurposeExpresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.DepositorInsertdonor plasmid targeting sgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHeEf1a-mEGFP-NLS
Plasmid#205012PurposeThe vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-JNKKTRClover
Plasmid#59139PurposeORF encoding JNK KTR mClover flanked by Gateway sequencesDepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
Available SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)
Plasmid#70191PurposeDerived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.DepositorInsertClpX (clpX )
Available SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only