Skip to main content
Addgene
Showing: 1 - 20 of 497 results

  1. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Fill out our guest blogger form to get started!   Thanks for all your hard work!   Fluorophores When is...Casilio is no longer available through Addgene.  Thanks again to all of our fantastic depositors and guest...

  3. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    ...contributed by the Developmental Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding...immunoprecipitation and more. The Developmental Studies Hybridoma Bank (DSHB), directed by David R. Soll, is a repository...Development (NICHD) in 1986, to enable basic research by banking and distributing monoclonal antibodies at cost....quality-control and technical support to the community. Thanks to Our Guest Blogger!   David Soll has been the... Director of the Developmental Studies Hybridoma Bank (DSHB) since 1996.       More Blog Posts about Scientific...

  5. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...fused to a fluorescent protein. “Frankenbody,” like the infamous frankenstein, was constructed by grafting...called this probe the HA frankenbody (Zhao et al., 2019).  To develop HA frankenbody, the Stasevich lab tested...can you do with the HA frankenbody? Another large advantage of the HA frankenbody is that it remains bound...provides HA frankenbody the ability to bind nascent HA tagged proteins.  Lastly, the HA frankenbody can be ...properly once inside cells.  Developing the HA frankenbody To combat the issues with these antibody based...lab observed colocalization of GFP tagged HA frankenbody with an mCherry, 4xHA tagged histone protein ... in U2OS cells. They then showed that the HA frankenbody probe could also bind a 1xHA tagged histone protein...

  6. Western Blot

    Type
    Protocol
    ...the chamber of the SDS-PAGE gel tank. Pro-Tip Different SDS-PAGE gel tanks will have different gel orientations...and pipettes Spectrophotometer Heat block Mini gel tank chamber Power supply iBlot 2 Gel Transfer Device...µg/mL . In duplicate, dilute 10 µL of standard, blank, and lysate samples into 200 µL of BCA reagent in...the plate. Subtract the average absorbance of the blank from all of the samples. Plot a standard curve of...orientations. Refer to the instructions for your specific tank. Close the clamp. Prepare 1X running buffer as follows.... Mix well. Fill the chamber of the SDS-PAGE gel tank with the 1X running buffer. Load the samples into... prestained protein ladder. Place the lid on the tank and plug the electrode cords into the power supply...

  7. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...restriction enzymes, you want to choose enzymes that: Flank your insert, but do not cut within your insert Are...options, such as: Adding desired restriction sites to flank your insert : You can use PCR Based Cloning and ... allow you to produce a version of your insert flanked by restriction sites compatible with the recipient...enough to have multiple options for enzymes that flank your insert and will result in correct orientation...

  8. Centrifugation

    Type
    Protocol
    ...balanced number of samples, you may need to add a blank. Blanks are often an extra tube filled with water to...density than water, then you may need to fill the blank based on weight instead of volume. Pro-Tip For microfuge...

  9. DNA Quantification

    Type
    Protocol
    ... Tips Before measuring any samples, be sure to ‘blank’ the spectrophotometer using the solution the DNA...DNA is resuspended in, but with no DNA added. 'Blanking' measures the background inherent to the machine...

  10. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...Sharps container Biohazard waste container Fire blanket Fire extinguisher Guidelines BSL-1 Guidelines Before.... A sink, eyewash station, safety shower, fire blanket, and extinguisher are also required to be present...

  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Anti-Ankyrin-B [N105/13R] Ankyrin-B Human Mouse IgG2a 177494 Anti-Ankyrin-B [N105/17R] Ankyrin-B Human...Rat Mouse IgG2a 188222 Anti-Shank1 and Shank3 [N367/51R] Shank1 and Shank3 Rat Mouse IgG2a 188223 Anti-Synaptotagmin...Mouse IgG2a 222158 Shank1 [N22/18R] Shank1 Rat Mouse IgG2a 222159 Shank2 [N23B/9R] Shank2 Rat Mouse IgG2a...206727 Shank3 scFv [N69/46] N69/46 scFv Shank3 scaffold protein Rat Mouse 206728 Shank1 and Shank3 scFv ... Mouse 190523 Ankyrin-B scFv [N105/13] N105/13 scFv Ankyrin-B Human Mouse 190524 Ankyrin-B scFv [N105/... N105/17 scFv Ankyrin-B Human Mouse 190525 Ankyrin-G scFv [N106/20] N106/20 scFv Ankyrin-G Human Mouse...Mouse 190542 Pan-Shank scFv [N23B/49] N23B/49 scFv Pan-Shank Rat Mouse 190543 Shank2 scFv [N23B/6] N23B...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... 76809 PANK2 gRNA (BRDN0001149459) PANK2 hU6 Hallervorden-Spatz disease David Root 76810 PANK2 gRNA (BRDN0001162474...BRDN0001162474) PANK2 hU6 Hallervorden-Spatz disease David Root 76811 PANK2 gRNA (BRDN0001162362) PANK2 hU6 Hallervorden-Spatz...LRRK2 Parkinson's William Hahn 23583 pDONR223-PANK2 PANK2 Hallervorden-Spatz disease William Hahn 23620...Hallervorden-Spatz disease David Root 76812 PANK2 gRNA (BRDN0001144887) PANK2 hU6 Hallervorden-Spatz disease David...Ac5 Parkinson's Elisa Izaurralde 79516 5E26 (PANK2) PANK2 His, TEV polH Hallervorden-Spatz disease Cheryl...OPTN_Halo_C_allele OPTN Halo ALS Michael Ward 178145 PANK2_Halo_C_allele PANK2 Halo Hallervorden-Spatz disease Michael...U6 Parkinson's Frank Soldner 180433 pU6-pegRNA-LRRK2-G2019S-3b LRRK2 U6 Parkinson's Frank Soldner 180434...

  13. Rett Syndrome

    Type
    Collection
    ... line repository Tissue Banks (Link opens in a new window) Harvard Brain Bank (Link opens in a new window...Autism Brain Bank (Link opens in a new window) University of Maryland Brain & Tissue Bank Antibodies (...Gribnau Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional... (Link opens in a new window) PMID: 6638958 Kankirawatana et al. 2006. Early progressive encephalopathy...

  14. CRISPR Plasmids - Tagging

    Type
    Collection
    ...pFETCh_RERE PX458_RERE_1 RFXANK Human FLAG pFETCh_RFXANK PX458_RFXANK_1 PX458_RFXANK_2 RXRB Human FLAG pFETCh_RXRB... donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and gRNA target sites...

  15. Cre-lox system

    Type
    Collection
    ...same cell type allows for recombination of LoxP flanked DNA sequences. Use the search box below to find...targeting vector with Cre FNF (neo-selectable marker flanked by FRT sites) Mammalian Mombaerts 15509 pBS-M71...vector with IRES CreFNF (neo-selectable marker flanked by FRT sites) Mammalian Mombaerts 17408 Puro.Cre...Cre-ERT2 Tamoxifen-inducible Cre None Zebrafish Stankunas 82696 pCRE-iRFP670 Cre and iRFP670 PGK Mammalian...Torok-Storb 99249 pVAX1/mTyr-Cre Cre Tyrosinase Mammalian Blank 101242 pSin wPGK-Cre low-efficiency Cre PGK Mammalian... either side of a gene (called “floxing”, for “flanked by loxP”), will permit gene expression until Cre...interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration; See similar plasmid...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ... expression) - RFXANK regulatory factor X-associated ankyrin-containing protein ANKRA1, BLS, F14150_1,...superfamily, member 11 CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf TNFSF12 tumor necrosis factor (ligand...CD265, FEO, LOH18CR1, ODFR, OFE, OPTB7, OSTS, PDB2, RANK, TRANCER TNFRSF11B tumor necrosis factor receptor...member 13b BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, ZTNF4 TNFSF14 tumor necrosis factor (ligand...

  17. Antibody Guide

    Type
    Collection
    ...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...which controls for your antibody’s specificity. A blank (contains water or buffer instead of a biological...Visualization methods should employ positive, negative, and blank controls. Antibody Applications - Cell Sorting Methods...with only one antibody, for each antibody used. Blank controls in the form of sample buffer must also ...

  18. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... to have 50-80bp of homology arms on each side flanking the change you'd like to introduce. We use Ultramer...plasmid donor, with two homology arms on each side flanking your desired insertion or mutation. Each arms ...plasmid donor, with two homology arms on each side flanking your desired insertion or mutation. Each arm is...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...promoter for normalization. FMDV StopGo sequences flank the MCS for improved normalization. John Atkins ...expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (neo) Akaluc hPGK...expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian expression...
Showing: 1 - 20 of 497 results