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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8 results for: Ank

Showing: 1 - 8 of 8 results

  1. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... packaging genes gag , pol , and env . The LTRs flank all of these required genes, and anything in between...contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging capacity Packaging plasmid... vectors are useful research and clinical tools thanks to their long-term expression of a transgene. Stable...generation of large-scale, high-titer preps. This is thanks to established retroviral production cell lines...-competent gamma-retroviral vectors is very low thanks to the design of the plasmids. The genes used for...

  2. Antibody Guide

    Type
    Guide
    ...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...which controls for your antibody’s specificity. A blank (contains water or buffer instead of a biological...Visualization methods should employ positive, negative, and blank controls. Antibody Applications - Cell Sorting Methods...with only one antibody, for each antibody used. Blank controls in the form of sample buffer must also ...

  3. Adenovirus Guide

    Type
    Guide
    ...and contain multiple heavily-spliced transcripts flanked by two inverted terminal repeats (ITRs). Adenoviral...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293 or HER911...genome packaging. In helper viruses, this signal is flanked by loxP sites so they can be excised. Adenoviral...

  4. Lentiviral Vector Guide

    Type
    Guide
    ... vif , vpr , vpu , and nef for HIV-1). The LTRs flank all of these genes, and anything in between will...contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging capacity Packaging plasmid...replication-competent lentiviral vectors is very low thanks to the design of second- and third-generation lentiviral...

  5. CRISPR Guide

    Type
    Guide
    ...large scale edits by integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated.... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby... PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...

  6. Plan Your Experiment

    Type
    Guide
    ...can locate potential PAM and target sequences and rank the associated gRNAs based on their predicted on-target...interest can be PCR amplified using primers that (A) flank the region of interest (deletions or small insertions...

  7. Cloning

    Type
    Guide
    ... The entry clone now has recombined attL sites flanking your DNA fragment of interest. Now that your DNA...
Showing: 1 - 8 of 8 results