...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...which controls for your antibody’s specificity. A blank (contains water or buffer instead of a biological...Visualization methods should employ positive, negative, and blank controls. Antibody Applications - Cell Sorting Methods...with only one antibody, for each antibody used. Blank controls in the form of sample buffer must also ...

... insert of interest. The transgene sequence is flanked by long terminal repeat (LTR) sequences, which ... between vectors until the gene of interest is flanked by sites compatible with those in the vector into... first cloned into an entry vector at a region flanked by sequences (called attP1 and attP2) that allow...

...large scale edits by integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated.... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby... PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...

...can locate potential PAM and target sequences and rank the associated gRNAs based on their predicted on-target...interest can be PCR amplified using primers that (A) flank the region of interest (deletions or small insertions...

...linearized with PacI to create a linear dsDNA construct flanked by ITRs. 293 or 911 cells are transfected with ...

...or shRNA of interest: The transgene sequence is flanked by long terminal repeat (LTR) sequences, which ...

...consists of two open reading frames, Rep and Cap, flanked by two 145 base inverted terminal repeats (ITRs...

... The entry clone now has recombined attL sites flanking your DNA fragment of interest. Now that your DNA...