...the chamber of the SDS-PAGE gel tank. Pro-Tip Different SDS-PAGE gel tanks will have different gel orientations...and pipettes Spectrophotometer Heat block Mini gel tank chamber Power supply iBlot 2 Gel Transfer Device...µg/mL . In duplicate, dilute 10 µL of standard, blank, and lysate samples into 200 µL of BCA reagent in...the plate. Subtract the average absorbance of the blank from all of the samples. Plot a standard curve of...orientations. Refer to the instructions for your specific tank. Close the clamp. Prepare 1X running buffer as follows.... Mix well. Fill the chamber of the SDS-PAGE gel tank with the 1X running buffer. Load the samples into... prestained protein ladder. Place the lid on the tank and plug the electrode cords into the power supply...

...restriction enzymes, you want to choose enzymes that: Flank your insert, but do not cut within your insert Are...options, such as: Adding desired restriction sites to flank your insert : You can use PCR Based Cloning and ... allow you to produce a version of your insert flanked by restriction sites compatible with the recipient...enough to have multiple options for enzymes that flank your insert and will result in correct orientation...

...balanced number of samples, you may need to add a blank. Blanks are often an extra tube filled with water to...density than water, then you may need to fill the blank based on weight instead of volume. Pro-Tip For microfuge...

... Tips Before measuring any samples, be sure to ‘blank’ the spectrophotometer using the solution the DNA...DNA is resuspended in, but with no DNA added. 'Blanking' measures the background inherent to the machine...

...Sharps container Biohazard waste container Fire blanket Fire extinguisher Guidelines BSL-1 Guidelines Before.... A sink, eyewash station, safety shower, fire blanket, and extinguisher are also required to be present...

...one or more unique oligonucleotide primers that flank the regions of your plasmid that you wish to confirm...

...ultracentrifugation. The steps are clearly distinguishable thanks to the use of phenol red. Image adapted from Zolotukhin...

...Select the Insert tab, then Chart . This will add a blank chart to your spreadsheet. In the Chart Editor ,...

...oligos from section B contain the shRNA sequence flanked by sequences that are compatible with the sticky...