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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,837 results for: pET

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Showing: 6441 - 6460 of 9837 results

  2. DNMT3B (3QKJ)

    Plasmid
    #32044
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DNMT3B (DNMT3B Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acids 293-442)
    Available Since
    Feb. 22, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. NCOR2

    Plasmid
    #40737
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NCOR2 (NCOR2 Human)
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Residues 615 to 685 of the SANT2 domain of Nuclea…
    Available Since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pMCRW1

    Plasmid
    #122462
    Purpose
    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
    Depositor
    Richard C. Willson
    Article
    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10
    Insert
    NPM-ALK fusion protein (ALK, NPM1 Human)
    Tags
    His Tag
    Expression
    Bacterial
    Promoter
    T7lac promoter
    Available Since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    122462-plasmid-map-sequence-id-243544

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  7. pLenti PGK mKate2-OGT 5N5A (Puro)

    Plasmid
    #154293
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; HCF-…
    Promoter
    PGK
    Available Since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154293-plasmid-map-sequence-id-307161

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  8. p28BIOHTEV-LIC

    Plasmid
    #167852
    Purpose
    Bacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purification
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Available Since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167852-plasmid-map-sequence-id-331065

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  13. KE-009

    Plasmid
    #133441
    Purpose
    Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4H (EIF4H Human)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133441-plasmid-map-sequence-id-267720

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  16. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

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  20. ARF5

    Plasmid
    #25530
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ARF5 (ARF5 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 6441 - 6460 of 9837 results